Somatic embryogenesis of Sandalwood (Santalum album L.)

• Published in: Indonesian Journal of Biotechnology
• Main Authors: Toni Herawan, Mohammad Na'iem, Sapto Indrioko, Ari Indrianto
• Format: Article
• Language: English
• Published: Universitas Gadjah Mada, Yogyakarta 2016-02-01
• Subjects: Somatic embryogenesis, Santalum album, Embryogenic callus, maturation
• Online Access: http://journal.ugm.ac.id/ijbiotech/article/view/9311
• ISSN: 0853-8654; 2089-2241

Sandalwood (Santalum album L.) is native species of Indonesia, especially in East Nusa Tenggara, is one<br />of the twenty two species of the genus Santalum in the world. Sandalwood is an important tree because it has<br />high economic value can produce sandal oil these can be used for perfumes, cosmetics, pharmaceuticals, and<br />are often used in religious ceremonies. In vitro particularly somatic embryogenesis has been widely applied<br />in the propagation of sandalwood. The Objective of this research is to obtain regeneration of sandalwood<br />through somatic embryogenesis using leaves explant from various clones. Medium for embryo induction is MS<br />(Murashige and Skoog, 1962) solid medium containing treatment of 2,4-D (2,4-Dichlorophenoxyacetic acid)<br />at various concentrations. To the media 0,15 mg /l kinetin, 40 g/l sucrose, and 2,5 g/l gelrite were added.<br />Culture were incubated in the dark. Medium for Embryo development (maturation) is MS solid medium<br />containing treatment of BAP (Benzyl-amino-purine) at various concentrations. To the media 0,01 mg /l NAA<br />(Napthalene-acetic-acid), 40 g/l sucrose, and 2,5 g/l gelrite were added. Culture were incubated in the light. To<br />study the specifi c structure of sandalwood somatic embryo early detection was conducted using histological<br />analysis. Results of anova showed that the clones, media, and interaction between clones with media did not<br />signifi cantly affect the development of sandalwood callus percentage. Results of anova showed that the clones<br />and BAP concentration signifi cantly effect to the embryo development of sandalwood.