Cre-assisted fine-mapping of neural circuits using orthogonal split inteins

Existing genetic methods of neuronal targeting do not routinely achieve the resolution required for mapping brain circuits. New approaches are thus necessary. Here, we introduce a method for refined neuronal targeting that can be applied iteratively. Restriction achieved at the first step can be fur...

Full description

Bibliographic Details
Published in:	eLife
Main Authors:	Haojiang Luan, Alexander Kuzin, Ward F Odenwald, Benjamin H White
Format:	Article
Language:	English
Published:	eLife Sciences Publications Ltd 2020-04-01
Subjects:	circuit mapping neuroblast Gal4-UAS proboscis extension dopamine expression system
Online Access:	https://elifesciences.org/articles/53041

Similar Items

Habituation, sensitization and Pavlovian conditioning
by: Münire Özlem Çevik
Published: (2014-02-01)

A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS
by: Brandon Mark, et al.
Published: (2021-05-01)

Downregulation of Plasma Membrane Ca2+ ATPase driven by tyrosine hydroxylase-Gal4 reduces Drosophila lifespan independently of effects in neurons
by: Brenda Erhardt, et al.
Published: (2023-12-01)

Associative Learning of Quantitative Mechanosensory Stimuli in Honeybees
by: Heather Strelevitz, et al.
Published: (2024-02-01)

No effect of low-level chronic neonicotinoid exposure on bumblebee learning and fecundity
by: Saija Piiroinen, et al.
Published: (2016-03-01)

Low-Level Fluvalinate Treatment in the Larval Stage Induces Impaired Olfactory Associative Behavior of Honey Bee Workers in the Field
by: Chong-Yu Ko, et al.
Published: (2022-03-01)

Unilateral Abscence Of Nose With Proboscis
by: Dr. Vikram Parkash Gupta, et al.
Published: (1991-02-01)

Engineered Genetic Circuits Activated by Bezafibrate Improve ESC‐Based TAA Cancer Vaccine Efficacy and PD‐L1 Nanobody Therapy
by: Meiling Jin, et al.
Published: (2025-06-01)

Stage-specific modulation of Drosophila gene expression with muscle GAL4 promoters
by: Ziwei Zhao, et al.
Published: (2025-12-01)

Combined Toxic Effects of Lead and Glyphosate on <i>Apis cerana cerana</i>
by: Yunfei Xue, et al.
Published: (2024-08-01)

Cyclopia: A Rare Congenital Anomaly
by: Uttara Gautam, et al.
Published: (2023-12-01)

Utilizing Drosophila Melanogaster in Alzheimer's Disease Research:Applications and Insights
by: Huiying Ma, et al.
Published: (2024-03-01)

Trap-TRAP, a Versatile Tool for Tissue-Specific Translatomics in Zebrafish
by: Jorge Corbacho, et al.
Published: (2022-01-01)

Controlling motor neurons of every muscle for fly proboscis reaching
by: Claire E McKellar, et al.
Published: (2020-06-01)

The NuA4/TIP60 histone-modifying complex and Hr78 modulate the Lobe2 mutant eye phenotype
by: Stonbraker Madison P., et al.
Published: (2025-08-01)

A direct-drive GFP reporter for studies of tracheal development in Drosophila
by: Geanette Lam, et al.
Published: (2022-12-01)

Establishment of a Novel Baculovirus–Silkworm Expression System
by: Junhong Wei, et al.
Published: (2022-05-01)

Enhancement of Vivid-based photo-activatable Gal4 transcription factor in mammalian cells
by: Shinji C. Nagasaki, et al.
Published: (2022-12-01)

Chronic Cadmium Exposure Induces Impaired Olfactory Learning and Altered Brain Gene Expression in Honey Bees (<i>Apis mellifera</i>)
by: Zhiguo Li, et al.
Published: (2022-10-01)

Honeybee Cognition as a Tool for Scientific Engagement
by: Jai A. Denton, et al.
Published: (2021-09-01)

The Gut–Brain–Microbiome Axis in Bumble Bees
by: Laura Leger, et al.
Published: (2020-08-01)

Cyclopia with proboscis: A rare congenital anomaly
by: Asma Kunwar, et al.
Published: (2021-07-01)

NUTRITION OF PROBOSCIS MONKEYS (NASALIS LARVATUS) FEED SOURCES AT BAKUT ISLAND NATURE PARK
by: Pribadi A.J., et al.
Published: (2022-11-01)

Drosophila medulla neuroblast termination via apoptosis, differentiation, and gliogenic switch is scheduled by the depletion of the neuroepithelial stem cell pool
by: Phuong-Khanh Nguyen, et al.
Published: (2024-06-01)

A repressor-decay timer for robust temporal patterning in embryonic Drosophila neuroblast lineages
by: Inna Averbukh, et al.
Published: (2018-12-01)

Rbp4-Gal4, a germline driver that activates in meiosis, reveals functions for VCP in spermatid development
by: Tyler J. Butsch, et al.
Published: (2023-12-01)

<i>Drosophila</i> as a Model of Unconventional Translation in Spinocerebellar Ataxia Type 3
by: Sean L. Johnson, et al.
Published: (2022-04-01)

A gene-specific T2A-GAL4 library for Drosophila
by: Pei-Tseng Lee, et al.
Published: (2018-03-01)

Learning performance and brain structure of artificially-reared honey bees fed with different quantities of food
by: Karin Steijven, et al.
Published: (2017-10-01)

Transplanted deep-layer cortical neuroblasts integrate into host neural circuits and alleviate motor defects in hypoxic-ischemic encephalopathy injured mice
by: Mengnan Wu, et al.
Published: (2024-11-01)

Single nuclei RNA-sequencing of adult brain neurons derived from type 2 neuroblasts reveals transcriptional complexity in the insect central complex
by: Derek G Epiney, et al.
Published: (2025-05-01)

A Case of Ethmocephaly in a Clomiphene Citrate Induced Pregnancy: A Case Report and Literature Review
by: Musie Negasi Gebreslase, et al.
Published: (2025-10-01)

Proboscis lateralis: A rare case report and brief literature review
by: Hisham Alshwaikh, et al.
Published: (2025-12-01)

Ultrastructural and Descriptive Study on the Adult Body Surface of <i>Heortia vitessoides</i> (Lepidoptera: Crambidae)
by: Lei Liu, et al.
Published: (2023-08-01)

Pseudocnidae of ribbon worms (Nemertea): ultrastructure, maturation, and functional morphology
by: Timur Yu Magarlamov, et al.
Published: (2021-02-01)

The Mouthparts of Female Blood-Feeding Frog-Biting Midges (Corethrellidae, Diptera)
by: Stephan Barton, et al.
Published: (2023-05-01)

Ventricular Ganglioneuroblastoma in an Adult and Successful Treatment with Radiotherapy
by: Mustafa Akın, et al.
Published: (2014-06-01)

Postnatal Changes of Somatostatin Expression in Hippocampi of C57BL/6 Mice; Modulation of Neuroblast Differentiation in the Hippocampus
by: Dae Young Yoo, et al.
Published: (2023-01-01)

Neuron hemilineages provide the functional ground plan for the Drosophila ventral nervous system
by: Robin M Harris, et al.
Published: (2015-07-01)

Symbionts on the Brain: How Wolbachia Is Strictly Corralled in Some Neotropical Drosophila spp.
by: Denis Voronin, et al.
Published: (2022-08-01)