Diversity and potential function of pig gut DNA viruses

• Published in: Heliyon
• Main Authors: Junjun Qin, Bingzhen Ji, Yijia Ma, Xin Liu, Tian Wang, Guiming Liu, Bugao Li, Guoliang Wang, Pengfei Gao
• Format: Article
• Language: English
• Published: Elsevier 2023-03-01
• Subjects: Pig gut; Viral metagenome; Viral diversity; Antibiotic resistance genes; Auxiliary metabolic genes
• Online Access: http://www.sciencedirect.com/science/article/pii/S2405844023012276

Viruses are ubiquitous in the gut of animals and play an important role in the ecology of the gut microbiome. The potential effects of these substances on the growth and development of the body are not fully known. Little is known about the effects of breeding environment on pig gut virome. Here, there are 3584 viral operational taxonomic units (vOTUs) longer than 5 kb identified by virus-enriched metagenome sequencing from 25 pig fecal samples. Only a small minority of vOTUs (11.16%) can be classified at the family level, and ∼50% of the genes could be annotated, supporting the concept of pig gut as reservoirs of substantial undescribed viral genetic diversity. The composition of pig gut virome in the six regions may be related to geography. There are only 20 viral clusters (VCs) shared among pig gut virome in six regions of Shanxi Province. These viruses rarely carry antibiotic resistance genes (ARGs). At the same time, they possess abundant auxiliary metabolic genes (AMGs) potentially involved in carbon, sulfur metabolism and cofactor biosynthesis, etc. This study has revealed the unique characteristics and potential function of pig gut DNA virome and established a foundation for the recognition of the viral roles in gut environment.