Isolation and manipulation of protoplasts from the unicellular green alga Penium margaritaceum

Abstract Background The unicellular charophycean green alga Penium margaritaceum has emerged as an appealing experimental organism in plant cell wall and cell biology research. Innovative practical approaches in the manipulation and maintenance of this unicellular model alga are needed in order to p...

Full description

Bibliographic Details
Published in:	Plant Methods
Main Authors:	Sandra Cristina Raimundo, Iben Sørensen, Berke Tinaz, Eleanore Ritter, Jocelyn K. C. Rose, David S. Domozych
Format:	Article
Language:	English
Published:	BMC 2018-02-01
Subjects:	Protoplasts Cellulase Pectate lyase Penium margaritaceum Nuclei isolation Intracellular markers
Online Access:	http://link.springer.com/article/10.1186/s13007-018-0284-9

Similar Items

Penium margaritaceum: A Unicellular Model Organism for Studying Plant Cell Wall Architecture and Dynamics
by: David S. Domozych
Published: (2014-11-01)

Experimental Manipulation of Pectin Architecture in the Cell Wall of the Unicellular Charophyte, Penium Margaritaceum
by: Kattia Palacio-Lopez, et al.
Published: (2020-07-01)

Charophytes: Evolutionary Giants and Emerging Model Organisms
by: David Domozych, et al.
Published: (2016-10-01)

The Secretome and N-Glycosylation Profiles of the Charophycean Green Alga, Penium margaritaceum, Resemble Those of Embryophytes
by: Eliel Ruiz-May, et al.
Published: (2018-03-01)

Enhancement of cellulolytic enzyme production from intrageneric protoplast fusion of Aspergillus species and evaluating the hydrolysate scavenging activity
by: Doaa A. Goda, et al.
Published: (2024-03-01)

An efficient method for protoplast-mediated production of transformed castor bean (Ricinus communis) lines
by: Paula Figueroa-Varela, et al.
Published: (2023-07-01)

Genome-Wide Identification of the Pectate Lyase Gene Family in Potato and Expression Analysis under Salt Stress
by: Zhiqi Wang, et al.
Published: (2024-05-01)

Hyperinduction of pectate lyase in Dickeya chrysanthemi EC16 by plant-derived sugars
by: Tri Joko, et al.
Published: (2018-01-01)

Genome-wide identification of the pectate lyase (PEL) gene family members in Malvaceae, and their contribution to cotton fiber quality
by: Qian Deng, et al.
Published: (2024-10-01)

Cotton Pectate Lyase GhPEL48_Dt Promotes Fiber Initiation Mediated by Histone Acetylation
by: Anlin Zhong, et al.
Published: (2024-08-01)

Characterization of a Novel Creeping Tartary Buckwheat (Fagopyrum tataricum) Mutant lazy1
by: Chenggang Liang, et al.
Published: (2022-04-01)

A Phloem-Expressed PECTATE LYASE-LIKE Gene Promotes Cambium and Xylem Development
by: Max Bush, et al.
Published: (2022-04-01)

Characterization and Functional Analysis of the Poplar Pectate Lyase-Like Gene PtPL1-18 Reveal Its Role in the Development of Vascular Tissues
by: Yun Bai, et al.
Published: (2017-06-01)

Genome-wide identification and expression analyses of the pectate lyase (PL) gene family in Fragaria vesca
by: Xiaolong Huang, et al.
Published: (2023-08-01)

Plant Breeding Through Protoplast Fusion
by: Devi Armita
Published: (2020-12-01)

A PL1 family pectate lyase CP966_RS08110 gene was the pathogenic factor of Streptomyces galilaeus 5T-1 causing potato common scab
by: Cuiwen Zhang, et al.
Published: (2024-11-01)

Double CRISPR knockout of pectin degrading enzymes improves tomato shelf‐life while ensuring fruit quality
by: Isabel Ortega‐Salazar, et al.
Published: (2024-03-01)

Rice <i>RBH1</i> Encoding A Pectate Lyase is Critical for Apical Panicle Development
by: Dong He, et al.
Published: (2021-01-01)

Pectate Lyase from Fusarium sacchari Induces Plant Immune Responses and Contributes to Virulence
by: Caixia Wang, et al.
Published: (2023-06-01)

Pectate Lyase Genes Abundantly Expressed During the Infection Regulate Morphological Development of Colletotrichum camelliae and CcPEL16 Is Required for Full Virulence to Tea Plants
by: Hong Jiang, et al.
Published: (2023-02-01)

Protoplast Isolation in Lupin Lupinus spp
by: Necmi Beşer, et al.
Published: (1996-05-01)

Protoplast cultures and protoplast fusion focused on Brassicaceae: A review
by: B. Navrátilová
Published: (2004-12-01)

Computational screening of walnut (Juglans regia) husk metabolites reveals Aesculin as a potential inhibitor of pectate lyase Pel3: Insights from molecular dynamics and τRAMD
by: Ali Khakpour, et al.
Published: (2025-09-01)

Characterization and Expression Analysis of Four Cadmium-Tolerance-Associated Genes of <i>Avicennia marina</i> (Forsk.)
by: Jinfeng Yu, et al.
Published: (2023-01-01)

Improvement of optimum pH and specific activity of pectate lyase from Bacillus RN.1 using loop replacement
by: Piwu Li, et al.
Published: (2023-07-01)

The Role of OmpR in the Expression of Genes of the KdgR Regulon Involved in the Uptake and Depolymerization of Oligogalacturonides in Yersinia enterocolitica
by: Marta Nieckarz, et al.
Published: (2017-08-01)

Isolation and culture of suspension protoplasts of vetiver
by: Somporn Prasertsongskun
Published: (2005-05-01)

Direct leaf-peeling method for areca protoplasts: a simple and efficient system for protoplast isolation and transformation in areca palm (Areca catechu)
by: Yaodi Wang, et al.
Published: (2023-01-01)

Isolation and culture of Celosia cristata L cell suspension protoplasts
by: Retno Mastuti, et al.
Published: (2003-06-01)

Molecular Characterization of a Novel Alkaline Endo-Pectate Lyase from <i>Paenibacillus borealis</i> and Over-Production in Bioreactor Realized by Constructing the Tandem Expression Cassettes in Host Genome
by: Ying Han, et al.
Published: (2025-09-01)

Genome-wide identification and expression analyses of the pectate lyase (PEL) gene family in cotton (Gossypium hirsutum L.)
by: Huiru Sun, et al.
Published: (2018-09-01)

Optimization of Protoplast Preparation and Regeneration of a Medicinal Fungus Antrodia cinnamomea
by: Jyun-De Wu, et al.
Published: (2019-10-01)

Locus TUTOU2 determines the panicle apical abortion phenotype of rice (Oryza sativa L.) in tutou2 mutant
by: Zi-chao ZHU, et al.
Published: (2022-03-01)

Pectate lyase is a factor in the adaptability for Heterodera glycines infecting tobacco
by: Zhong-ling TIAN, et al.
Published: (2019-03-01)

Novel Phage-Derived Depolymerase with Activity against <i>Proteus mirabilis</i> Biofilms
by: Cormac J. Rice, et al.
Published: (2021-10-01)

Rational design and structure-based engineering of alkaline pectate lyase from Paenibacillus sp. 0602 to improve thermostability
by: Zhanping Zhou, et al.
Published: (2021-05-01)

Isolation of berry skin protoplasts and improvement of their produce
by: K. Amrani Joutei, et al.
Published: (1996-03-01)

Isolation and culture of protoplast from leaves of Lactuca sativa
by: Witool Chaipakdee
Published: (2007-07-01)

Dissecting the molecular dance: c-di-GMP, cAMP-CRP, and VfmH collaboration in pectate lyase regulation for Dickeya dadantii—unveiling the soft rot pathogen’s strategy
by: Biswarup Banerjee, et al.
Published: (2023-12-01)

Transcriptome Analysis of Sugarcane Young Leaves and Protoplasts after Enzymatic Digestion
by: Demei Zhang, et al.
Published: (2022-08-01)