Separation of Sedative – Hypnotic Drugs with Mixed Micellar Liquid Chromatography
Separation of ten sedative- hypnotic drugs was performed by RP-HPLC using mixed micellar mobile phase. Effect of temperature, type and amount of organic modifier in mobile phase on efficiency (N) and asymmetry factor (B/A) showed that, the appropriate conditions for a good separation were 35°C and 7...
| Published in: | Iranian Journal of Chemistry & Chemical Engineering |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
| Published: |
Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR
2005-06-01
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| Subjects: | |
| Online Access: | http://www.ijcce.ac.ir/article_8125_21bad6dc9f08544be2c8bbf6f4693115.pdf |
| Summary: | Separation of ten sedative- hypnotic drugs was performed by RP-HPLC using mixed micellar mobile phase. Effect of temperature, type and amount of organic modifier in mobile phase on efficiency (N) and asymmetry factor (B/A) showed that, the appropriate conditions for a good separation were 35°C and 7% (V/V) butanol in mobile phase. Variations of selectivity factor versus butanol concentration, Mixture of SDS/ Brij- 35 concentrations and pH of mobile phase showed that the appropriate conditions were 7%(V/V), 15 mM SDS +1.0mM Brij-35 and pH=3 in mobile phase respectively. |
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| ISSN: | 1021-9986 1021-9986 |