| Summary: | Municipal greenery can mitigate the negative impact of urbanization on biodiversity, including bats, by providing a migration corridor, food base and roosts. Our study aimed to evaluate the species composition and diversity, test the differences in activity between seasons, and identify the atmospheric conditions influencing the bats’ activity in the Planty Park (Cracow). Fieldworks were conducted in 2016 and 2017. We recorded 10 species, two new for this part of Poland: the Kuhl’s pipistrelle (<i>Pipistrellus kuhlii</i>) and the Savi’s pipistrelle (<i>Hypsugo savii</i>). Taxa were divided into three ecological guilds. <i>Myotis</i> group’s activity was insufficient to perform statistical analyses. The activity of <i>Nyctalus</i>, <i>Eptesicus</i> and <i>Vespertilio</i> group peaked in late summer. A similar insignificant trend was observed for <i>Pipistrellus</i> and <i>Hypsugo</i>. Temperature enhanced the activity of <i>Nyctalus</i>, <i>Eptesicus</i> and <i>Vespertilio</i> group in spring and early summer, while cloud cover suppressed their activity in autumn. Temperature also enhanced <i>Pipistrellus</i> and <i>Hypsugo</i> group activity in spring and autumn, but it suppressed their summer activity. Our study is one of the first to investigate temperate urban bats’ phenology and may serve as a preface for further research to introduce detailed urban landscape planning recommendations.
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