Non-Polio Enterovirus C Replicate in Both Airway and Intestine Organotypic Cultures
Non-polio enteroviruses (EV) belonging to species C, which are highly prevalent in Africa, mainly among children, are poorly characterized, and their pathogenesis is mostly unknown as they are difficult to culture. In this study, human airway and intestinal organotypic models were used to investigat...
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| Format: | Article |
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2023-08-01
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| Online Access: | https://www.mdpi.com/1999-4915/15/9/1823 |
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| author | Giulia Moreni Hetty van Eijk Gerrit Koen Nina Johannesson Carlemi Calitz Kimberley Benschop Jeroen Cremer Dasja Pajkrt Adithya Sridhar Katja Wolthers |
| author_facet | Giulia Moreni Hetty van Eijk Gerrit Koen Nina Johannesson Carlemi Calitz Kimberley Benschop Jeroen Cremer Dasja Pajkrt Adithya Sridhar Katja Wolthers |
| author_sort | Giulia Moreni |
| collection | DOAJ |
| container_title | Viruses |
| description | Non-polio enteroviruses (EV) belonging to species C, which are highly prevalent in Africa, mainly among children, are poorly characterized, and their pathogenesis is mostly unknown as they are difficult to culture. In this study, human airway and intestinal organotypic models were used to investigate tissue and cellular tropism of three EV-C genotypes, EV-C99, CVA-13, and CVA-20. Clinical isolates were obtained within the two passages of culture on Caco2 cells, and all three viruses were replicated in both the human airway and intestinal organotypic cultures. We did not observe differences in viral replication between fetal and adult tissue that could potentially explain the preferential infection of infants by EV-C genotypes. Infection of the airway and the intestinal cultures indicates that they both can serve as entry sites for non-polio EV-C. Ciliated airway cells and enterocytes are the target of infection for all three viruses, as well as enteroendocrine cells for EV-C99. |
| format | Article |
| id | doaj-art-d2acbd6b261a481dbce99bbf05bb9161 |
| institution | Directory of Open Access Journals |
| issn | 1999-4915 |
| language | English |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| spelling | doaj-art-d2acbd6b261a481dbce99bbf05bb91612025-08-19T22:40:38ZengMDPI AGViruses1999-49152023-08-01159182310.3390/v15091823Non-Polio Enterovirus C Replicate in Both Airway and Intestine Organotypic CulturesGiulia Moreni0Hetty van Eijk1Gerrit Koen2Nina Johannesson3Carlemi Calitz4Kimberley Benschop5Jeroen Cremer6Dasja Pajkrt7Adithya Sridhar8Katja Wolthers9OrganoVIR Labs, Department of Medical Microbiology, Amsterdam UMC, Location AMC, Amsterdam Institute for Infection and Immunity, University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsOrganoVIR Labs, Department of Medical Microbiology, Amsterdam UMC, Location AMC, Amsterdam Institute for Infection and Immunity, University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsOrganoVIR Labs, Department of Medical Microbiology, Amsterdam UMC, Location AMC, Amsterdam Institute for Infection and Immunity, University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsOrganoVIR Labs, Department of Medical Microbiology, Amsterdam UMC, Location AMC, Amsterdam Institute for Infection and Immunity, University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsOrganoVIR Labs, Department of Medical Microbiology, Amsterdam UMC, Location AMC, Amsterdam Institute for Infection and Immunity, University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsNational Institute for Public Health and Environment, RIVM, 3721 MA Bilthoven, The NetherlandsNational Institute for Public Health and Environment, RIVM, 3721 MA Bilthoven, The NetherlandsOrganoVIR Labs, Department of Pediatric Infectious Diseases, Emma Children’s Hospital, Amsterdam UMC, Location AMC, University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsOrganoVIR Labs, Department of Medical Microbiology, Amsterdam UMC, Location AMC, Amsterdam Institute for Infection and Immunity, University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsOrganoVIR Labs, Department of Medical Microbiology, Amsterdam UMC, Location AMC, Amsterdam Institute for Infection and Immunity, University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsNon-polio enteroviruses (EV) belonging to species C, which are highly prevalent in Africa, mainly among children, are poorly characterized, and their pathogenesis is mostly unknown as they are difficult to culture. In this study, human airway and intestinal organotypic models were used to investigate tissue and cellular tropism of three EV-C genotypes, EV-C99, CVA-13, and CVA-20. Clinical isolates were obtained within the two passages of culture on Caco2 cells, and all three viruses were replicated in both the human airway and intestinal organotypic cultures. We did not observe differences in viral replication between fetal and adult tissue that could potentially explain the preferential infection of infants by EV-C genotypes. Infection of the airway and the intestinal cultures indicates that they both can serve as entry sites for non-polio EV-C. Ciliated airway cells and enterocytes are the target of infection for all three viruses, as well as enteroendocrine cells for EV-C99.https://www.mdpi.com/1999-4915/15/9/1823non-polio enterovirusenterovirus C speciestropismCVA-13CVA-20EV-C99 |
| spellingShingle | Giulia Moreni Hetty van Eijk Gerrit Koen Nina Johannesson Carlemi Calitz Kimberley Benschop Jeroen Cremer Dasja Pajkrt Adithya Sridhar Katja Wolthers Non-Polio Enterovirus C Replicate in Both Airway and Intestine Organotypic Cultures non-polio enterovirus enterovirus C species tropism CVA-13 CVA-20 EV-C99 |
| title | Non-Polio Enterovirus C Replicate in Both Airway and Intestine Organotypic Cultures |
| title_full | Non-Polio Enterovirus C Replicate in Both Airway and Intestine Organotypic Cultures |
| title_fullStr | Non-Polio Enterovirus C Replicate in Both Airway and Intestine Organotypic Cultures |
| title_full_unstemmed | Non-Polio Enterovirus C Replicate in Both Airway and Intestine Organotypic Cultures |
| title_short | Non-Polio Enterovirus C Replicate in Both Airway and Intestine Organotypic Cultures |
| title_sort | non polio enterovirus c replicate in both airway and intestine organotypic cultures |
| topic | non-polio enterovirus enterovirus C species tropism CVA-13 CVA-20 EV-C99 |
| url | https://www.mdpi.com/1999-4915/15/9/1823 |
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