Cu-TCPP-Mn nanozymes composite platelet-rich plasma hydrogel for infected wound healing

• Published in: Materials & Design
• Main Authors: Han Chen, Pu Yang, Yikun Ju, Songjie Li, Xin Dan, Ping Xue, Xuanji Huang, Lanjie Lei, Xing Fan, Yang Li
• Format: Article
• Language: English
• Published: Elsevier 2025-11-01
• Subjects: Cu-TCPP-Mn nanozyme; Hydrogel; Platelet-rich plasma; Wound healing
• Online Access: http://www.sciencedirect.com/science/article/pii/S0264127525012742

The management of infectious wounds is still a critical clinical challenge. This study introduces a multifunctional hydrogel composed of oxidized hyaluronic acid (OHA) and carboxymethyl chitosan (CMCS), enhanced with platelet-rich plasma (PRP) and copper-tetra(4-carboxyphenyl)porphyrin-manganese (Cu-TCPP-Mn) nanozymes for advanced wound-healing applications. The hydrogel was synthesized via a Schiff base reaction, crosslinking the aldehyde groups of OHA with the carboxymethyl groups of CMCS to create a dynamic network with tunable rheological properties and excellent biocompatibility. Incorporation of PRP enriched the hydrogel with growth factors, markedly promoting tissue regeneration, whereas Cu-TCPP-Mn nanozymes effectively scavenged reactive oxygen species by mimicking superoxide dismutase and catalase activities. Furthermore, the hydrogel modulated macrophage polarization, and the synergistic effects of PRP and Cu-TCPP-Mn helped to alleviate persistent inflammation in infected wounds. A series of experiments showed that the CHPM hydrogel enhanced cell proliferation, migration, and differentiation, modulated inflammatory responses, and accelerated wound healing. The hydrogel also exhibited superior swelling capacity, self-healing behavior, and shape-memory functionality. These results underscore the potential of this hydrogel to be an innovative bioactive dressing for infectious wound healing with substantial promise for clinical antibacterial applications.