| الملخص: | Polysaccharides are crucial dietary supplements and traditional pharmacological components of <i>Ganoderma lucidum</i>; however, the mechanisms responsible for high polysaccharide yields in <i>G. lucidum</i> remain unclear. Therefore, we investigated the mechanisms underlying the high yield of polysaccharides in submerged cultures of <i>G. lucidum</i> using transcriptomic and proteomic analyses. Several glycoside hydrolase (GH) genes and proteins, which are associated with the degradation of fungal cell walls, were significantly upregulated under high polysaccharide yield conditions. They mainly belonged to the GH3, GH5, GH16, GH17, GH18, GH55, GH79, GH128, GH152, and GH154 families. Additionally, the results suggested that the cell wall polysaccharide could be degraded by GHs, which is beneficial for extracting more intracellular polysaccharides from cultured mycelia. Furthermore, some of the degraded polysaccharides were released into the culture broth, which is beneficial for obtaining more extracellular polysaccharides. Our findings provide new insights into the mechanisms underlying the roles that GH family genes play to regulate high polysaccharide yields in <i>G. lucidum</i>.
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