Lyso-IP: Uncovering Pathogenic Mechanisms of Lysosomal Dysfunction
Lysosomes are ubiquitous membrane-bound organelles found in all eukaryotic cells. Outside of their well-known degradative function, lysosomes are integral in maintaining cellular homeostasis. Growing evidence has shown that lysosomal dysfunction plays an important role not only in the rare group of...
| Published in: | Biomolecules |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
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| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/12/5/616 |
| _version_ | 1850415094385606656 |
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| author | Chase Chen Ellen Sidransky Yu Chen |
| author_facet | Chase Chen Ellen Sidransky Yu Chen |
| author_sort | Chase Chen |
| collection | DOAJ |
| container_title | Biomolecules |
| description | Lysosomes are ubiquitous membrane-bound organelles found in all eukaryotic cells. Outside of their well-known degradative function, lysosomes are integral in maintaining cellular homeostasis. Growing evidence has shown that lysosomal dysfunction plays an important role not only in the rare group of lysosomal storage diseases but also in a host of others, including common neurodegenerative disorders, such as Alzheimer disease and Parkinson disease. New technological advances have significantly increased our ability to rapidly isolate lysosomes from cells in recent years. The development of the Lyso-IP approach and similar methods now allow for lysosomal purification within ten minutes. Multiple studies using the Lyso-IP approach have revealed novel insights into the pathogenic mechanisms of lysosomal disorders, including Niemann-Pick type C disease, showing the immense potential for this technique. Future applications of rapid lysosomal isolation techniques are likely to greatly enhance our understanding of lysosomal dysfunction in rare and common neurodegeneration causes. |
| format | Article |
| id | doaj-art-d8e119bef21c4f5891bfe8ccc366b2ca |
| institution | Directory of Open Access Journals |
| issn | 2218-273X |
| language | English |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| spelling | doaj-art-d8e119bef21c4f5891bfe8ccc366b2ca2025-08-19T22:45:18ZengMDPI AGBiomolecules2218-273X2022-04-0112561610.3390/biom12050616Lyso-IP: Uncovering Pathogenic Mechanisms of Lysosomal DysfunctionChase Chen0Ellen Sidransky1Yu Chen2Section on Molecular Neurogenetics, Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bld 35A, Room 1E623 35 Convent Drive, MSC 3708, Rockville, MD 20892, USASection on Molecular Neurogenetics, Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bld 35A, Room 1E623 35 Convent Drive, MSC 3708, Rockville, MD 20892, USASection on Molecular Neurogenetics, Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bld 35A, Room 1E623 35 Convent Drive, MSC 3708, Rockville, MD 20892, USALysosomes are ubiquitous membrane-bound organelles found in all eukaryotic cells. Outside of their well-known degradative function, lysosomes are integral in maintaining cellular homeostasis. Growing evidence has shown that lysosomal dysfunction plays an important role not only in the rare group of lysosomal storage diseases but also in a host of others, including common neurodegenerative disorders, such as Alzheimer disease and Parkinson disease. New technological advances have significantly increased our ability to rapidly isolate lysosomes from cells in recent years. The development of the Lyso-IP approach and similar methods now allow for lysosomal purification within ten minutes. Multiple studies using the Lyso-IP approach have revealed novel insights into the pathogenic mechanisms of lysosomal disorders, including Niemann-Pick type C disease, showing the immense potential for this technique. Future applications of rapid lysosomal isolation techniques are likely to greatly enhance our understanding of lysosomal dysfunction in rare and common neurodegeneration causes.https://www.mdpi.com/2218-273X/12/5/616lysosomeslysosome isolationNiemann-Pick type C |
| spellingShingle | Chase Chen Ellen Sidransky Yu Chen Lyso-IP: Uncovering Pathogenic Mechanisms of Lysosomal Dysfunction lysosomes lysosome isolation Niemann-Pick type C |
| title | Lyso-IP: Uncovering Pathogenic Mechanisms of Lysosomal Dysfunction |
| title_full | Lyso-IP: Uncovering Pathogenic Mechanisms of Lysosomal Dysfunction |
| title_fullStr | Lyso-IP: Uncovering Pathogenic Mechanisms of Lysosomal Dysfunction |
| title_full_unstemmed | Lyso-IP: Uncovering Pathogenic Mechanisms of Lysosomal Dysfunction |
| title_short | Lyso-IP: Uncovering Pathogenic Mechanisms of Lysosomal Dysfunction |
| title_sort | lyso ip uncovering pathogenic mechanisms of lysosomal dysfunction |
| topic | lysosomes lysosome isolation Niemann-Pick type C |
| url | https://www.mdpi.com/2218-273X/12/5/616 |
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