Molecular Characterization of Novel x-Type HMW Glutenin Subunit 1B × 6.5 in Wheat

• Published in: Plants
• Main Authors: Tímea Kuťka Hlozáková, Zdenka Gálová, Svetlana Šliková, Leona Leišová-Svobodová, Jana Beinhauer, Filip Dyčka, Marek Šebela, Erika Zetochová, Edita Gregová
• Format: Article
• Language: English
• Published: MDPI AG 2021-10-01
• Subjects: wheat; HMW glutenin; 1-D electrophoresis; 2-D electrophoresis; mass spectrometry
• Online Access: https://www.mdpi.com/2223-7747/10/10/2108
• ISSN: 2223-7747

A novel high molecular weight glutenin subunit encoded by the Glu-1B locus was identified in the French genotype Bagou, which we named 1B × 6.5. This subunit differed in SDS-PAGE from well-known 1B × 6 and 1B × 7 subunits, which are also encoded at this locus. Subunit 1B × 6.5 has a theoretical molecular weight of 88,322.83 Da, which is more mobile than 1B × 6 subunit, and isoelectric point (pI) of about 8.7, which is lower than that for 1B × 6 subunit. The specific primers were designed to amplify and sequence 2476 bp of the Glu-1B locus from genotype Bagou. A high level of similarity was found between the sequence encoding 1B × 6.5 and other x-type encoding alleles of this locus.