Development of Recombinase Polymerase Amplification Combined with Lateral Flow Strips for Rapid Detection of Cowpea Mild Mottle Virus

• الحاوية / القاعدة: The Plant Pathology Journal
• المؤلفون الرئيسيون: Xinyang Wu, Shuting Chen, Zixin Zhang, Yihan Zhang, Pingmei Li, Xinyi Chen, Miaomiao Liu, Qian Lu, Zhongyi Li, Zhongyan Wei, Pei Xu
• التنسيق: مقال
• اللغة: الإنجليزية
• منشور في: Hanrimwon Publishing Company 2023-10-01
• الموضوعات: cowpea mild mottle virus; rapid on-field detection; sensitivity
• الوصول للمادة أونلاين: http://ppjonline.org/upload/pdf/PPJ-OA-02-2023-0033.pdf
• تدمد: 1598-2254; 2093-9280

Cowpea mild mottle virus (CPMMV) is a global plant virus that poses a threat to the production and quality of legume crops. Early and accurate diagnosis is essential for effective managing CPMMV outbreaks. With the advancement in isothermal recombinase polymerase amplification and lateral flow strips technologies, more rapid and sensitive methods have become available for detecting this pathogen. In this study, we have developed a reverse transcription recombinase polymerase amplification combined with lateral flow strips (RT-RPA-LFS) method for the detection of CPMMV, specifically targeting the CPMMV coat protein (CP) gene. The RT-RPA-LFS assay only requires 20 min at 40°C and demonstrates high specificity. Its detection limit was 10 copies/μl, which is approximately up to 100 times more sensitive than RT-PCR on agarose gel electrophoresis. The developed RT-RPA-LFS method offers a rapid, convenient, and sensitive approach for field detection of CPMMV, which contribute to controlling the spread of the virus.