High‐Throughput Parallel Optofluidic 3D‐Imaging Flow Cytometry
3D light‐sheet microscopy is a powerful tool to obtain content‐rich information of cell populations. However, the limited frame rate of high‐quantum‐efficiency cameras hinders its application in high‐throughput flow cytometry. Herein, a high‐throughput 3D‐imaging flow cytometry technique based on li...
| 发表在: | Small Science |
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| Main Authors: | , |
| 格式: | 文件 |
| 语言: | 英语 |
| 出版: |
Wiley-VCH
2022-07-01
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| 主题: | |
| 在线阅读: | https://doi.org/10.1002/smsc.202100126 |
| _version_ | 1852662448210837504 |
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| author | Masashi Ugawa Sadao Ota |
| author_facet | Masashi Ugawa Sadao Ota |
| author_sort | Masashi Ugawa |
| collection | DOAJ |
| container_title | Small Science |
| description | 3D light‐sheet microscopy is a powerful tool to obtain content‐rich information of cell populations. However, the limited frame rate of high‐quantum‐efficiency cameras hinders its application in high‐throughput flow cytometry. Herein, a high‐throughput 3D‐imaging flow cytometry technique based on light‐sheet microscopy that can screen thousands of cells per second is introduced. This method enables fast, parallel optofluidic scanning of cells by performing 1D acoustofluidic focusing on multiple cells under wide‐field light‐sheet microscopy with a single objective lens. Multicolor 3D‐imaging flow cytometry with cell‐line samples at a record detection throughput of over 2000 cells s−1 is demonstrated. Furthermore, an unprecedentedly large‐scale 3D‐morphology‐based flow cytometric analysis at an order of 105 cells is demonstrated. Results show that the system is able to capture subcellular structures even at high throughputs and obtain cellular information that is overlooked under 2D‐imaging cytometry. |
| format | Article |
| id | doaj-art-dc0aaf9e5b6a4381a4da8e2ae2f207f0 |
| institution | Directory of Open Access Journals |
| issn | 2688-4046 |
| language | English |
| publishDate | 2022-07-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| spelling | doaj-art-dc0aaf9e5b6a4381a4da8e2ae2f207f02025-08-19T21:36:19ZengWiley-VCHSmall Science2688-40462022-07-0127n/an/a10.1002/smsc.202100126High‐Throughput Parallel Optofluidic 3D‐Imaging Flow CytometryMasashi Ugawa0Sadao Ota1Research Center for Advanced Science and Technology The University of Tokyo 4-6-1 Komaba, Meguro-ku Tokyo 153-8904 JapanResearch Center for Advanced Science and Technology The University of Tokyo 4-6-1 Komaba, Meguro-ku Tokyo 153-8904 Japan3D light‐sheet microscopy is a powerful tool to obtain content‐rich information of cell populations. However, the limited frame rate of high‐quantum‐efficiency cameras hinders its application in high‐throughput flow cytometry. Herein, a high‐throughput 3D‐imaging flow cytometry technique based on light‐sheet microscopy that can screen thousands of cells per second is introduced. This method enables fast, parallel optofluidic scanning of cells by performing 1D acoustofluidic focusing on multiple cells under wide‐field light‐sheet microscopy with a single objective lens. Multicolor 3D‐imaging flow cytometry with cell‐line samples at a record detection throughput of over 2000 cells s−1 is demonstrated. Furthermore, an unprecedentedly large‐scale 3D‐morphology‐based flow cytometric analysis at an order of 105 cells is demonstrated. Results show that the system is able to capture subcellular structures even at high throughputs and obtain cellular information that is overlooked under 2D‐imaging cytometry.https://doi.org/10.1002/smsc.202100126acoustofluidic particle focusingimaging flow cytometrylight-sheet microscopy |
| spellingShingle | Masashi Ugawa Sadao Ota High‐Throughput Parallel Optofluidic 3D‐Imaging Flow Cytometry acoustofluidic particle focusing imaging flow cytometry light-sheet microscopy |
| title | High‐Throughput Parallel Optofluidic 3D‐Imaging Flow Cytometry |
| title_full | High‐Throughput Parallel Optofluidic 3D‐Imaging Flow Cytometry |
| title_fullStr | High‐Throughput Parallel Optofluidic 3D‐Imaging Flow Cytometry |
| title_full_unstemmed | High‐Throughput Parallel Optofluidic 3D‐Imaging Flow Cytometry |
| title_short | High‐Throughput Parallel Optofluidic 3D‐Imaging Flow Cytometry |
| title_sort | high throughput parallel optofluidic 3d imaging flow cytometry |
| topic | acoustofluidic particle focusing imaging flow cytometry light-sheet microscopy |
| url | https://doi.org/10.1002/smsc.202100126 |
| work_keys_str_mv | AT masashiugawa highthroughputparalleloptofluidic3dimagingflowcytometry AT sadaoota highthroughputparalleloptofluidic3dimagingflowcytometry |