Generation of ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC double reporter line for monitoring of pancreatic differentiation

Pancreatic islets consist of several different endocrine cell types that work in harmony. Aside from primary pancreatic islets, stem cell-derived pancreatic islets can be used as an alternative research and disease model. Here, we introduce a double reporter line of ARX-T2A-H2B-CFP x C-PEP-mCherry-h...

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Bibliographic Details
Published in:Stem Cell Research
Main Authors: Eunike Sawitning Ayu Setyono, Nicole Katarina Rogers, Anita Hofmann, Heiko Lickert, Ingo Burtscher
Format: Article
Language:English
Published: Elsevier 2025-04-01
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506125000352
Description
Summary:Pancreatic islets consist of several different endocrine cell types that work in harmony. Aside from primary pancreatic islets, stem cell-derived pancreatic islets can be used as an alternative research and disease model. Here, we introduce a double reporter line of ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC through CRISPR/Cas9-mediated insertion of mCherry in the C-terminus of C-Peptide in the previously published ARX-CFP hiPSC line. This reporter line allows live monitoring of stem cell-derived pancreatic alpha and beta cells throughout differentiation.
ISSN:1873-5061

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