Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays

Abstract Background An efficient in vivo transient transfection system using protoplasts is an important tool to study gene expression, metabolic pathways, and multiple mutagenesis parameters in plants. Although rice protoplasts can be isolated from germinated seedlings or cell suspension culture, p...

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Bibliographic Details
Published in:	Plant Methods
Main Authors:	Snigdha Poddar, Jaclyn Tanaka, Jamie H. D. Cate, Brian Staskawicz, Myeong-Je Cho
Format:	Article
Language:	English
Published:	BMC 2020-11-01
Subjects:	Protoplast isolation Calli Pause point Transfection Genome editing assay Rice
Online Access:	http://link.springer.com/article/10.1186/s13007-020-00692-4

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