Comparison of Universal mtDNA Primers in Species Identification of Animals in a Sample with Severely Degraded DNA

Analysis of mitochondrial DNA, specifically the cytochrome b gene (cyt b), has become an essential tool for species identification. In the case of degraded samples, in which DNA is fractionated, universal primers, which are highly effective at amplifying the target region, are necessary. The materia...

Full description

Bibliographic Details
Published in:	Animals
Main Authors:	Aleksandra Figura, Magdalena Gryzinska, Andrzej Jakubczak
Format:	Article
Language:	English
Published:	MDPI AG 2024-11-01
Subjects:	mitochondrial DNA DNA barcoding cytochrome b 12S rRNA 16S rRNA species identification
Online Access:	https://www.mdpi.com/2076-2615/14/22/3256

Similar Items

Phylogenetic systematics of Barn Owl (Tyto alba (Scopoli, 1769)) complex inferred from mitochondrial rDNA (16S rRNA) taxonomic implication
by: Mansour Aliabadian, et al.
Published: (2012-09-01)

High degree of mitochondrial gene heterogeneity in the bat tick species Ixodes vespertilionis, I. ariadnae and I. simplex from Eurasia
by: Sándor Hornok, et al.
Published: (2015-09-01)

DNA barcoding of Cynoglossus arel using mitochondrial COI and 16S rRNA genes
by: CHITRA SOMAN, et al.
Published: (2020-10-01)

Genetic differentiation of Macrodon ancylodon (Sciaenidae, Perciformes) populations in Atlantic coastal waters of South America as revealed by mtDNA analysis
by: Simôni Santos, et al.
Published: (2003-01-01)

Paleoparasitological report on Ascaris aDNA from an ancient East Asian sample
by: Chang Seok Oh, et al.
Published: (2010-03-01)

Comparing sediment DNA extraction methods for assessing organic enrichment associated with marine aquaculture
by: John K. Pearman, et al.
Published: (2020-10-01)

Typical structure of rRNA coding genes in diplonemids points to two independent origins of the bizarre rDNA structures of euglenozoans
by: Paweł Hałakuc, et al.
Published: (2022-05-01)

Polimorfismo do 16S mtDNA por LIS-SSCP em Melipona scutellaris Latreille, 1811 (Hymenoptera, Apidae, Meliponini)
by: Ana Carolina Siquieroli, et al.
Published: (2006-05-01)

Sampling technique to pool genetic materials of microorganism communities in blue-swimming crab processing plant industry
by: Asadatun Abdullah, et al.
Published: (2024-06-01)

Occurrence of Sturnira tildae De La Torre, 1959 (Chiroptera: Phyllostomidae) in the state of Maranhão, Brazil
by: Amanda Cristiny da Silva Lima, et al.
Published: (2021-10-01)

Identification and phylogenetic analysis of marine sponges in the Jordanian Gulf of Aqaba using DNA barcoding
by: Zeinab Arabeyyat, et al.
Published: (2025-02-01)

Comparison of two commercial DNA extraction kits for the analysis of nasopharyngeal bacterial communities
by: Keith A. Crandall, et al.
Published: (2016-04-01)

DNA barcoding of reef‐associated fishes of Saint Martin's Island, Northern Bay of Bengal, Bangladesh
by: Kazi Ahsan Habib, et al.
Published: (2023-10-01)

Comparison of four DNA extraction methods for 16s rRNA microbiota profiling of human faecal samples
by: James Sinclair, et al.
Published: (2023-08-01)

Quatrefoil light traps for free-swimming stages of cymothoid parasitic isopods and seasonal variation in their species compositions in the Seto Inland Sea, Japan
by: Hiroki Fujita, et al.
Published: (2023-04-01)

Increased risk of hearing loss associated with MT-RNR1 gene mutations: a real-world investigation among Han Taiwanese Population
by: Hou-Kuang Chen, et al.
Published: (2024-06-01)

Characterization of prokaryotic plankton community structure in the Southern East China Sea using combined 16S-rDNA and 16S-rRNA
by: Yi-Ting Chang, et al.
Published: (2025-08-01)

Species distribution and screening of Trypanosoma DNA in phlebotomine sand flies from four southern provinces of Thailand
by: Nantatchaporn Klaiklueng, et al.
Published: (2025-01-01)

DNA metabarcoding assessment of Neotropical ichthyoplankton communities is marker‐dependent
by: Daniel Fonseca Teixeira, et al.
Published: (2023-10-01)

Ants of French Guiana: 16S rRNA sequence dataset
by: Gaëtan Rongier, et al.
Published: (2023-02-01)

Taxonomic implications of molecular studies on Northern Brazilian Teredinidae (Mollusca: Bivalvia) specimens
by: Sonia Maria Lima Santos, et al.
Published: (2005-03-01)

DIAGNOSIS OF BACTEREMIA USING UNIVERSAL PCR IN FEBRILE ILL CHILDREN
by: Afsharpaiman, et al.
Published: (2007-04-01)

Developing the script “degenerate primer 111” to enhance the coverage of universal primers for the small subunit rRNA gene on target microorganisms
by: Zhihui Qin, et al.
Published: (2024-09-01)

Identification and distribution of Rhipicephalus microplus in selected high-cattle density districts in Uganda: signaling future demand for novel tick control approaches
by: Patrick Etiang, et al.
Published: (2024-03-01)

Rhizosphere microbiomes of field-grown B. stricta exhibit minimal diel changes in microbial membership and protein synthesis potential
by: Alessandra Ceretto, et al.
Published: (2025-09-01)

An Improved Methodology to Overcome Key Issues in Human Fecal Metagenomic DNA Extraction
by: Jitendra Kumar, et al.
Published: (2016-12-01)

Discovery of previously unrecorded goby Bathygobius cyclopterus (Valenciennes, 1837) (Perciformes: Gobiidae) from Jeju Island, Korea, inferred from mitochondrial DNA sequences
by: Hyuck Joon Kwun
Published: (2021-09-01)

Analysis of the Holarctic <em>Dictyoptera aurora</em> Complex (Coleoptera, Lycidae) Reveals Hidden Diversity and Geographic Structure in Müllerian Mimicry Ring
by: Michal Motyka, et al.
Published: (2022-09-01)

Impact of preservation method and storage period on ribosomal metabarcoding of marine microbes: Implications for remote automated samplings
by: Matthias Wietz, et al.
Published: (2022-09-01)

Moving environmental DNA (eDNA) technologies from benchtop to the field using passive sampling and PDQeX extraction
by: Gert‐Jan Jeunen, et al.
Published: (2022-11-01)

In Vitro Passive eDNA Sampling Provides a Cost‐Effective Alternative for Large Scale Sample Collection
by: Samuel Thompson, et al.
Published: (2025-05-01)

DNA-elucidated life cycle of a highly pathogenic avian nematode: Streptocara incognita (Spirurida: Acuariidae) and its morphological development from infective third-stage larva to adult
by: Yasen Mutafchiev, et al.
Published: (2025-01-01)

Evaluation of Established Methods for DNA Extraction and Primer Pairs Targeting 16S rRNA Gene for Bacterial Microbiota Profiling of Olive Xylem Sap
by: Carmen Haro, et al.
Published: (2021-03-01)

Bog bacterial community: data from north-western Russia
by: Ivan Zubov, et al.
Published: (2024-08-01)

The rDNA Diversity, Interseasonal Dynamic, and Functional Role of Cyanobacteria <i>Synechococcus</i> in the Sub-Arctic White Sea
by: Tatiana A. Belevich, et al.
Published: (2024-11-01)

eDNA Metabarcoding Analysis of Bony Fish Identification in Coastal Water of Gulf of Maine Using Nested Polymerase Chain Reaction of 12S rRNA Universal Primers
by: Bo‐Young Lee, et al.
Published: (2024-11-01)

Investigation of Detection Limits and the Influence of DNA Extraction and Primer Choice on the Observed Microbial Communities in Drinking Water Samples Using 16S rRNA Gene Amplicon Sequencing
by: Jakob Brandt, et al.
Published: (2018-09-01)

Reduced ribosomal RNA expression and unchanged ribosomal DNA promoter methylation in oral squamous cell carcinoma
by: Shanshan Ha, et al.
Published: (2019-07-01)

Evaluation of DNA extraction methods and direct PCR in metabarcoding of mock and marine bacterial communities
by: Iva Stojan, et al.
Published: (2023-04-01)

UPDATE OF THE MOLECULAR PHYLOGENY OF THE AUSTROPOTAMOBIUS PALLIPES SPECIES COMPLEX BY INCLUDING SPECIMENS FROM SOUTH TYROL (ITALY) AND CARINTHIA (AUSTRIA)
by: BARIC S., et al.
Published: (2005-07-01)