| 總結: | Due to recent outbreaks of cyclosporiasis associated with consumption of fresh berries, producers are demanding modern microbiological tools for the rapid and accurate identification of the human pathogen <i>Cyclospora cayetanensis</i> in berries and environmental samples. The aim of the present work was to develop a molecular tool based on a PCR approach for the rapid and accurate detection of <i>C. cayetanensis</i>. A nested PCR assay was validated for the amplification of a 294 bp size region of the <i>18S rRNA</i> gene from <i>C. cayetanensis</i>. The limit of detection for the nested PCR assay was validated using 48 berry samples spiked with ~0, 10, 100, and 1000 oocyst per gram of sample. With this assay, it was possible to detect as few as 1 oocyst per gram of berry, in a 50 g sample. Sanger DNA sequencing and phylogenetic analysis were carried out to confirm the presence of <i>C. cayetanensis</i> in berry (n = 17) and soil (n = 5) samples. The phylogenetic analysis revealed that the <i>C. cayetanensis</i> sequences obtained from Mexico clustered within a group recovered from China, Peru, Guatemala-Haiti, and Japan. The PCR protocol designed in the present study could be an important tool for the rapid and accurate detection of this human pathogen in environmental and food samples.
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