Transcriptome‐wide analysis of circRNA and RBP profiles and their molecular relevance for GBM
Glioblastoma (GBM) is the most aggressive and lethal type of glioma, characterized by aberrant expression of noncoding RNAs including circular RNAs (circRNAs). CircRNAs may impact cellular processes by interacting with other molecules—like RNA‐binding proteins (RBPs). The diagnostic value of circRNA...
| الحاوية / القاعدة: | Molecular Oncology |
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| المؤلفون الرئيسيون: | , , , , , , , , , , , , , , |
| التنسيق: | مقال |
| اللغة: | الإنجليزية |
| منشور في: |
Wiley
2025-08-01
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://doi.org/10.1002/1878-0261.70005 |
| _version_ | 1849425874334515200 |
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| author | Julia Latowska‐Łysiak Żaneta Zarębska Marcin P. Sajek Adriana Grabowska Alessia Buratin Paweł Głodowicz Julia O. Misiorek Konrad Kuczyński Stefania Bortoluzzi Marek Żywicki Jan G. Kosiński Agnieszka Rybak‐Wolf Rafał Piestrzeniewicz Anna M. Barciszewska Katarzyna Rolle |
| author_facet | Julia Latowska‐Łysiak Żaneta Zarębska Marcin P. Sajek Adriana Grabowska Alessia Buratin Paweł Głodowicz Julia O. Misiorek Konrad Kuczyński Stefania Bortoluzzi Marek Żywicki Jan G. Kosiński Agnieszka Rybak‐Wolf Rafał Piestrzeniewicz Anna M. Barciszewska Katarzyna Rolle |
| author_sort | Julia Latowska‐Łysiak |
| collection | DOAJ |
| container_title | Molecular Oncology |
| description | Glioblastoma (GBM) is the most aggressive and lethal type of glioma, characterized by aberrant expression of noncoding RNAs including circular RNAs (circRNAs). CircRNAs may impact cellular processes by interacting with other molecules—like RNA‐binding proteins (RBPs). The diagnostic value of circRNA and circRNA/RBP complexes is still largely unknown. To explore circRNA and RBP transcript expression in GBM, we performed and further analyzed RNA‐seq data from GBM patients' primary and recurrent tumor samples. We identified circRNAs differentially expressed in primary tumors, the circRNA progression markers in recurrent GBM samples, and the expression profile of RBP genes. Furthermore, we demonstrated the clinical potential of circRNAs and RBPs in GBM and proposed them as stratification markers in de novo assembled tumor subtypes. Additionally, we experimentally validated the subcellular localization of select circRNAs and their interactions with FUS. Subsequently, we showed that circARID1A may play a role in promoting GBM cell proliferation. Overall, we described circRNA‐RBP interactions that could play a regulatory role in gliomagenesis and GBM progression and provided a list of molecular players in GBM for further extensive studies. |
| format | Article |
| id | doaj-art-e264f073dcd44082aa28887aae4f218b |
| institution | Directory of Open Access Journals |
| issn | 1574-7891 1878-0261 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Wiley |
| record_format | Article |
| spelling | doaj-art-e264f073dcd44082aa28887aae4f218b2025-08-20T03:41:11ZengWileyMolecular Oncology1574-78911878-02612025-08-011982270229110.1002/1878-0261.70005Transcriptome‐wide analysis of circRNA and RBP profiles and their molecular relevance for GBMJulia Latowska‐Łysiak0Żaneta Zarębska1Marcin P. Sajek2Adriana Grabowska3Alessia Buratin4Paweł Głodowicz5Julia O. Misiorek6Konrad Kuczyński7Stefania Bortoluzzi8Marek Żywicki9Jan G. Kosiński10Agnieszka Rybak‐Wolf11Rafał Piestrzeniewicz12Anna M. Barciszewska13Katarzyna Rolle14Department of Molecular Neurooncology Institute of Bioorganic Chemistry, Polish Academy of Sciences Poznań PolandDepartment of Molecular Neurooncology Institute of Bioorganic Chemistry, Polish Academy of Sciences Poznań PolandRNA Bioscience Initiative, University of Colorado School of Medicine Aurora CO USADepartment of Molecular Neurooncology Institute of Bioorganic Chemistry, Polish Academy of Sciences Poznań PolandDepartment of Molecular Medicine University of Padua ItalyDepartment of Molecular Neurooncology Institute of Bioorganic Chemistry, Polish Academy of Sciences Poznań PolandDepartment of Molecular Neurooncology Institute of Bioorganic Chemistry, Polish Academy of Sciences Poznań PolandFaculty of Chemistry Adam Mickiewicz University Poznań PolandDepartment of Molecular Medicine University of Padua ItalyDepartment of Computational Biology Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University Poznań PolandDepartment of Computational Biology Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University Poznań PolandOrganoid Platform, Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC) Berlin GermanyDepartment of Neurosurgery Józef Struś Hospital Poznań PolandIntraoperative Imaging Unit, Department of Neurosurgery and Neurotraumatology Poznań University of Medical Sciences Poznań PolandDepartment of Molecular Neurooncology Institute of Bioorganic Chemistry, Polish Academy of Sciences Poznań PolandGlioblastoma (GBM) is the most aggressive and lethal type of glioma, characterized by aberrant expression of noncoding RNAs including circular RNAs (circRNAs). CircRNAs may impact cellular processes by interacting with other molecules—like RNA‐binding proteins (RBPs). The diagnostic value of circRNA and circRNA/RBP complexes is still largely unknown. To explore circRNA and RBP transcript expression in GBM, we performed and further analyzed RNA‐seq data from GBM patients' primary and recurrent tumor samples. We identified circRNAs differentially expressed in primary tumors, the circRNA progression markers in recurrent GBM samples, and the expression profile of RBP genes. Furthermore, we demonstrated the clinical potential of circRNAs and RBPs in GBM and proposed them as stratification markers in de novo assembled tumor subtypes. Additionally, we experimentally validated the subcellular localization of select circRNAs and their interactions with FUS. Subsequently, we showed that circARID1A may play a role in promoting GBM cell proliferation. Overall, we described circRNA‐RBP interactions that could play a regulatory role in gliomagenesis and GBM progression and provided a list of molecular players in GBM for further extensive studies.https://doi.org/10.1002/1878-0261.70005circRNAGBMRBPRNA‐seq |
| spellingShingle | Julia Latowska‐Łysiak Żaneta Zarębska Marcin P. Sajek Adriana Grabowska Alessia Buratin Paweł Głodowicz Julia O. Misiorek Konrad Kuczyński Stefania Bortoluzzi Marek Żywicki Jan G. Kosiński Agnieszka Rybak‐Wolf Rafał Piestrzeniewicz Anna M. Barciszewska Katarzyna Rolle Transcriptome‐wide analysis of circRNA and RBP profiles and their molecular relevance for GBM circRNA GBM RBP RNA‐seq |
| title | Transcriptome‐wide analysis of circRNA and RBP profiles and their molecular relevance for GBM |
| title_full | Transcriptome‐wide analysis of circRNA and RBP profiles and their molecular relevance for GBM |
| title_fullStr | Transcriptome‐wide analysis of circRNA and RBP profiles and their molecular relevance for GBM |
| title_full_unstemmed | Transcriptome‐wide analysis of circRNA and RBP profiles and their molecular relevance for GBM |
| title_short | Transcriptome‐wide analysis of circRNA and RBP profiles and their molecular relevance for GBM |
| title_sort | transcriptome wide analysis of circrna and rbp profiles and their molecular relevance for gbm |
| topic | circRNA GBM RBP RNA‐seq |
| url | https://doi.org/10.1002/1878-0261.70005 |
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