Beneficial impacts of neuromuscular electrical stimulation on muscle structure and function in the zebrafish model of Duchenne muscular dystrophy
Neuromuscular electrical stimulation (NMES) allows activation of muscle fibers in the absence of voluntary force generation. NMES could have the potential to promote muscle homeostasis in the context of muscle disease, but the impacts of NMES on diseased muscle are not well understood. We used the z...
| 出版年: | eLife |
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| 主要な著者: | , , , , , , , , , , , , , , |
| フォーマット: | 論文 |
| 言語: | 英語 |
| 出版事項: |
eLife Sciences Publications Ltd
2022-03-01
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| 主題: | |
| オンライン･アクセス: | https://elifesciences.org/articles/62760 |
| _version_ | 1852695045677776896 |
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| author | Elisabeth A Kilroy Amanda C Ignacz Kaylee L Brann Claire E Schaffer Devon Varney Sarah S Alrowaished Kodey J Silknitter Jordan N Miner Ahmed Almaghasilah Tashawna L Spellen Alexandra D Lewis Karissa Tilbury Benjamin L King Joshua B Kelley Clarissa A Henry |
| author_facet | Elisabeth A Kilroy Amanda C Ignacz Kaylee L Brann Claire E Schaffer Devon Varney Sarah S Alrowaished Kodey J Silknitter Jordan N Miner Ahmed Almaghasilah Tashawna L Spellen Alexandra D Lewis Karissa Tilbury Benjamin L King Joshua B Kelley Clarissa A Henry |
| author_sort | Elisabeth A Kilroy |
| collection | DOAJ |
| container_title | eLife |
| description | Neuromuscular electrical stimulation (NMES) allows activation of muscle fibers in the absence of voluntary force generation. NMES could have the potential to promote muscle homeostasis in the context of muscle disease, but the impacts of NMES on diseased muscle are not well understood. We used the zebrafish Duchenne muscular dystrophy (dmd) mutant and a longitudinal design to elucidate the consequences of NMES on muscle health. We designed four neuromuscular stimulation paradigms loosely based on weightlifting regimens. Each paradigm differentially affected neuromuscular structure, function, and survival. Only endurance neuromuscular stimulation (eNMES) improved all outcome measures. We found that eNMES improves muscle and neuromuscular junction morphology, swimming, and survival. Heme oxygenase and integrin alpha7 are required for eNMES-mediated improvement. Our data indicate that neuromuscular stimulation can be beneficial, suggesting that the right type of activity may benefit patients with muscle disease. |
| format | Article |
| id | doaj-art-e30495404e4840359dd4fb9984b3b084 |
| institution | Directory of Open Access Journals |
| issn | 2050-084X |
| language | English |
| publishDate | 2022-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| spelling | doaj-art-e30495404e4840359dd4fb9984b3b0842025-08-19T21:22:59ZengeLife Sciences Publications LtdeLife2050-084X2022-03-011110.7554/eLife.62760Beneficial impacts of neuromuscular electrical stimulation on muscle structure and function in the zebrafish model of Duchenne muscular dystrophyElisabeth A Kilroy0Amanda C Ignacz1Kaylee L Brann2Claire E Schaffer3Devon Varney4Sarah S Alrowaished5Kodey J Silknitter6Jordan N Miner7https://orcid.org/0000-0003-2998-3330Ahmed Almaghasilah8Tashawna L Spellen9Alexandra D Lewis10Karissa Tilbury11Benjamin L King12Joshua B Kelley13Clarissa A Henry14https://orcid.org/0000-0001-7204-9231Graduate School of Biomedical Science and Engineering, University of Maine, Orono, United StatesGraduate School of Biomedical Science and Engineering, University of Maine, Orono, United StatesSchool of Biology and Ecology, University of Maine, Orono, United StatesSchool of Biology and Ecology, University of Maine, Orono, United StatesSchool of Biology and Ecology, University of Maine, Orono, United StatesSchool of Biology and Ecology, University of Maine, Orono, United StatesGraduate School of Biomedical Science and Engineering, University of Maine, Orono, United StatesDepartment of Chemical and Biomedical Engineering, University of Maine, Orono, United StatesGraduate School of Biomedical Science and Engineering, University of Maine, Orono, United StatesSchool of Biology and Ecology, University of Maine, Orono, United StatesSchool of Biology and Ecology, University of Maine, Orono, United StatesGraduate School of Biomedical Science and Engineering, University of Maine, Orono, United States; Department of Chemical and Biomedical Engineering, University of Maine, Orono, United StatesGraduate School of Biomedical Science and Engineering, University of Maine, Orono, United States; Department of Molecular and Biomedical Sciences, University of Maine, Orono, United StatesGraduate School of Biomedical Science and Engineering, University of Maine, Orono, United States; Department of Molecular and Biomedical Sciences, University of Maine, Orono, United StatesGraduate School of Biomedical Science and Engineering, University of Maine, Orono, United States; School of Biology and Ecology, University of Maine, Orono, United StatesNeuromuscular electrical stimulation (NMES) allows activation of muscle fibers in the absence of voluntary force generation. NMES could have the potential to promote muscle homeostasis in the context of muscle disease, but the impacts of NMES on diseased muscle are not well understood. We used the zebrafish Duchenne muscular dystrophy (dmd) mutant and a longitudinal design to elucidate the consequences of NMES on muscle health. We designed four neuromuscular stimulation paradigms loosely based on weightlifting regimens. Each paradigm differentially affected neuromuscular structure, function, and survival. Only endurance neuromuscular stimulation (eNMES) improved all outcome measures. We found that eNMES improves muscle and neuromuscular junction morphology, swimming, and survival. Heme oxygenase and integrin alpha7 are required for eNMES-mediated improvement. Our data indicate that neuromuscular stimulation can be beneficial, suggesting that the right type of activity may benefit patients with muscle disease.https://elifesciences.org/articles/62760neuromuscular developmentdystroglycanopathiesskeletal muscle |
| spellingShingle | Elisabeth A Kilroy Amanda C Ignacz Kaylee L Brann Claire E Schaffer Devon Varney Sarah S Alrowaished Kodey J Silknitter Jordan N Miner Ahmed Almaghasilah Tashawna L Spellen Alexandra D Lewis Karissa Tilbury Benjamin L King Joshua B Kelley Clarissa A Henry Beneficial impacts of neuromuscular electrical stimulation on muscle structure and function in the zebrafish model of Duchenne muscular dystrophy neuromuscular development dystroglycanopathies skeletal muscle |
| title | Beneficial impacts of neuromuscular electrical stimulation on muscle structure and function in the zebrafish model of Duchenne muscular dystrophy |
| title_full | Beneficial impacts of neuromuscular electrical stimulation on muscle structure and function in the zebrafish model of Duchenne muscular dystrophy |
| title_fullStr | Beneficial impacts of neuromuscular electrical stimulation on muscle structure and function in the zebrafish model of Duchenne muscular dystrophy |
| title_full_unstemmed | Beneficial impacts of neuromuscular electrical stimulation on muscle structure and function in the zebrafish model of Duchenne muscular dystrophy |
| title_short | Beneficial impacts of neuromuscular electrical stimulation on muscle structure and function in the zebrafish model of Duchenne muscular dystrophy |
| title_sort | beneficial impacts of neuromuscular electrical stimulation on muscle structure and function in the zebrafish model of duchenne muscular dystrophy |
| topic | neuromuscular development dystroglycanopathies skeletal muscle |
| url | https://elifesciences.org/articles/62760 |
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