Checkpoint phosphorylation sites on budding yeast Rif1 protect nascent DNA from degradation by Sgs1-Dna2.

In budding yeast the Rif1 protein is important for protecting nascent DNA at blocked replication forks, but the mechanism has been unclear. Here we show that budding yeast Rif1 must interact with Protein Phosphatase 1 to protect nascent DNA. In the absence of Rif1, removal of either Dna2 or Sgs1 pre...

Full description

Bibliographic Details
Published in:	PLoS Genetics
Main Authors:	Vamsi Krishna Gali, Chandre Monerawela, Yassine Laksir, Shin-Ichiro Hiraga, Anne D Donaldson
Format:	Article
Language:	English
Published:	Public Library of Science (PLoS) 2023-11-01
Online Access:	https://doi.org/10.1371/journal.pgen.1011044

Similar Items

Protection of nascent DNA at stalled replication forks is mediated by phosphorylation of RIF1 intrinsically disordered region
by: Sandhya Balasubramanian, et al.
Published: (2022-04-01)

The human RIF1-Long isoform interacts with BRCA1 to promote recombinational fork repair under DNA replication stress
by: Qianqian Dong, et al.
Published: (2025-07-01)

Extensive DNA end processing by exo1 and sgs1 inhibits break-induced replication.
by: Vanessa A Marrero, et al.
Published: (2010-07-01)

At short telomeres Tel1 directs early replication and phosphorylates Rif1.
by: Akila Sridhar, et al.
Published: (2014-10-01)

An anti-checkpoint activity for rif1.
by: Yaniv Harari, et al.
Published: (2011-12-01)

Heteroduplex DNA position defines the roles of the Sgs1, Srs2, and Mph1 helicases in promoting distinct recombination outcomes.
by: Katrina Mitchel, et al.
Published: (2013-01-01)

Shepherding DNA ends: Rif1 protects telomeres and chromosome breaks
by: Mario Alberto Flores-Valdez, et al.
Published: (2018-05-01)

Phosphorylation-dependent interactions between Crb2 and Chk1 are essential for DNA damage checkpoint.
by: Meng Qu, et al.
Published: (2012-07-01)

Mitochondrial Replication Assay (MIRA) for Efficient in situ Quantification of Nascent mtDNA and Protein Interactions with Nascent mtDNA (mitoSIRF)
by: Macy Lozen, et al.
Published: (2023-05-01)

The RIF1-long splice variant promotes G1 phase 53BP1 nuclear bodies to protect against replication stress
by: Lotte P Watts, et al.
Published: (2020-11-01)

ISG15 conjugation to proteins on nascent DNA mitigates DNA replication stress
by: Christopher P. Wardlaw, et al.
Published: (2022-10-01)

Compartmentalized DNA repair: Rif1 S-acylation links DNA double-strand break repair to the nuclear membrane
by: Gabriele A. Fontana, et al.
Published: (2019-11-01)

Phosphorylation-mediated interactions with TOPBP1 couple 53BP1 and 9-1-1 to control the G1 DNA damage checkpoint
by: Nicolas Bigot, et al.
Published: (2019-05-01)

The COMPASS subunit Spp1 protects nascent DNA at the Tus/Ter replication fork barrier by limiting DNA availability to nucleases
by: Nagham Ghaddar, et al.
Published: (2023-09-01)

Inactivation of the SGS1 and EXO1 Genes Synergistically Stimulates Plasmid Integration in Yeast
by: Anamarija Štafa, et al.
Published: (2005-01-01)

Rif1 Controls DNA Replication Timing in Yeast through the PP1 Phosphatase Glc7
by: Stefano Mattarocci, et al.
Published: (2014-04-01)

A Proteomic Characterization of Factors Enriched at Nascent DNA Molecules
by: Andres J. Lopez-Contreras, et al.
Published: (2013-04-01)

Direct and indirect control of the initiation of meiotic recombination by DNA damage checkpoint mechanisms in budding yeast.
by: Bilge Argunhan, et al.
Published: (2013-01-01)

Phosphorylation of the MBF repressor Yox1p by the DNA replication checkpoint keeps the G1/S cell-cycle transcriptional program active.
by: Catia Caetano, et al.
Published: (2011-02-01)

The nascent polypeptide-associated complex subunit Egd1 is required for efficient selective mitochondrial degradation in budding yeast
by: Yuan Tian, et al.
Published: (2024-01-01)

RIF1 integrates DNA repair and transcriptional requirements during the establishment of humoral immune responses
by: Eleni Kabrani, et al.
Published: (2025-01-01)

Rif1 Binding and Control of Chromosome-Internal DNA Replication Origins Is Limited by Telomere Sequestration
by: Lukas Hafner, et al.
Published: (2018-04-01)

SUBGLOTTIC STENOSIS (SGS) PASCA TRAUMA INHALASI
by: Jilvientasia Godive Lilihata, et al.
Published: (2021-12-01)

The roles of the Saccharomyces cerevisiae RecQ helicase SGS1 in meiotic genome surveillance.
by: Amit Dipak Amin, et al.
Published: (2010-11-01)

Delineation of the role of chromatin assembly and the Rtt101Mms1 E3 ubiquitin ligase in DNA damage checkpoint recovery in budding yeast.
by: Li-Ting Diao, et al.
Published: (2017-01-01)

Phosphorylation Targets of DNA-PK and Their Role in HIV-1 Replication
by: Andrey Anisenko, et al.
Published: (2020-08-01)

CtIP-dependent nascent RNA expression flanking DNA breaks guides the choice of DNA repair pathway
by: Daniel Gómez-Cabello, et al.
Published: (2022-09-01)

Author Correction: The nascent polypeptide-associated complex subunit Egd1 is required for efficient selective mitochondrial degradation in budding yeast
by: Yuan Tian, et al.
Published: (2024-04-01)

Nonenzymatic Role for WRN in Preserving Nascent DNA Strands after Replication Stress
by: Fengtao Su, et al.
Published: (2014-11-01)

Multi-step processing of replication stress-derived nascent strand DNA gaps by MRE11 and EXO1 nucleases
by: Anastasia Hale, et al.
Published: (2023-10-01)

Cyclin Kinase-independent role of p21CDKN1A in the promotion of nascent DNA elongation in unstressed cells
by: Sabrina F Mansilla, et al.
Published: (2016-10-01)

A mitotic phosphorylation feedback network connects Cdk1, Plk1, 53BP1, and Chk2 to inactivate the G(2)/M DNA damage checkpoint.
by: Marcel A T M van Vugt, et al.
Published: (2010-01-01)

S6K1 phosphorylates Cdk1 and MSH6 to regulate DNA repair
by: Adi Amar-Schwartz, et al.
Published: (2022-10-01)

Composition of media for in vitro slow growth storage (sgs) of Aglaonema
by: Pangesti Nugrahani, et al.
Published: (2024-04-01)

Reversal of DDK-Mediated MCM Phosphorylation by Rif1-PP1 Regulates Replication Initiation and Replisome Stability Independently of ATR/Chk1
by: Robert C. Alver, et al.
Published: (2017-03-01)

Correction: Essential and Checkpoint Functions of Budding Yeast ATM and ATR during Meiotic Prophase Are Facilitated by Differential Phosphorylation of a Meiotic Adaptor Protein, Hop1.
by: Ana Penedos, et al.
Published: (2016-01-01)

Smc5/6 Mediated Sumoylation of the Sgs1-Top3-Rmi1 Complex Promotes Removal of Recombination Intermediates
by: Jacob N. Bonner, et al.
Published: (2016-07-01)

Programmable DNA shell scaffolds for directional membrane budding
by: Michael T. Pinner, et al.
Published: (2025-10-01)

High plasticity of ribosomal DNA organization in budding yeast
by: Shuangying Jiang, et al.
Published: (2024-02-01)

What influences DNA replication rate in budding yeast?
by: Thomas W Spiesser, et al.
Published: (2010-01-01)