Rapid, simple, and effective strategy to produce monoclonal antibodies targeting protein structures using hybridoma technology

Highlights ・MIHS-SAST screening generates mAbs that target protein structures. ・One-fourth of the mAbs recognized stereospecific epitopes of the protein. ・Two fluorometric parameters aided in selecting antibodies with strong binding constants.

Bibliographic Details
Published in:	Journal of Biological Engineering
Main Authors:	Atsumi Sakaguchi, Yoichiro Tanaka, Eiki Shoji, Teppei Takeshima, Rina Sakamaki, Takao Matsuba, Yasuyuki Kurihara
Format:	Article
Language:	English
Published:	BMC 2023-03-01
Subjects:	Monoclonal antibody Flow cytometer Conformation-specific antibody ELISA Hybridoma technology
Online Access:	https://doi.org/10.1186/s13036-023-00345-9

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