Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins located in the sarcomere, cytoskeleton and the extracellular matrix
Sarcopenia of old age is characterized by the progressive loss of skeletal muscle mass and concomitant decrease in contractile strength. Age-related skeletal muscle dysfunctions play a key pathophysiological role in the frailty syndrome and can result in a drastically diminished quality of life in t...
| Published in: | European Journal of Translational Myology |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2024-05-01
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| Subjects: | |
| Online Access: | https://www.pagepressjournals.org/bam/article/view/12564 |
| _version_ | 1850385999715106816 |
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| author | Paul Dowling Stephen Gargan Margit Zweyer Michael Henry Paula Meleady Dieter Swandulla Kay Ohlendieck |
| author_facet | Paul Dowling Stephen Gargan Margit Zweyer Michael Henry Paula Meleady Dieter Swandulla Kay Ohlendieck |
| author_sort | Paul Dowling |
| collection | DOAJ |
| container_title | European Journal of Translational Myology |
| description | Sarcopenia of old age is characterized by the progressive loss of skeletal muscle mass and concomitant decrease in contractile strength. Age-related skeletal muscle dysfunctions play a key pathophysiological role in the frailty syndrome and can result in a drastically diminished quality of life in the elderly. Here we have used mass spectrometric analysis of the mouse hindlimb musculature to establish the muscle protein constellation at advanced age of a widely used sarcopenic animal model. Proteomic results were further analyzed by systems bioinformatics of voluntary muscles. In this report, the proteomic survey of aged muscles has focused on the expression patterns of proteins involved in the contraction-relaxation cycle, membrane cytoskeletal maintenance and the formation of the extracellular matrix. This includes proteomic markers of the fast versus slow phenotypes of myosin-containing thick filaments and actin-containing thin filaments, as well as proteins that are associated with the non-sarcomeric cytoskeleton and various matrisomal layers. The bioanalytical usefulness of the newly established reference map was demonstrated by the comparative screening of normal versus dystrophic muscles of old age, and findings were verified by immunoblot analysis.
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| format | Article |
| id | doaj-art-e471eb9effea4ca5bcd3bad522cd5177 |
| institution | Directory of Open Access Journals |
| issn | 2037-7452 2037-7460 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| spelling | doaj-art-e471eb9effea4ca5bcd3bad522cd51772025-08-19T22:55:35ZengPAGEPress PublicationsEuropean Journal of Translational Myology2037-74522037-74602024-05-0110.4081/ejtm.2024.12564Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins located in the sarcomere, cytoskeleton and the extracellular matrixPaul Dowling0Stephen Gargan1Margit Zweyer2Michael Henry3Paula Meleady4Dieter Swandulla5Kay Ohlendieck6Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland; Kathleen Lonsdale Institute for Human Health Research, Maynooth University, Maynooth, Co. KildareDepartment of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland; Kathleen Lonsdale Institute for Human Health Research, Maynooth University, Maynooth, Co. KildareDepartment of Neonatology and Paediatric Intensive Care, Children’s Hospital, University of Bonn, Bonn, Germany; German Center for Neurodegenerative Diseases, BonnNational Institute for Cellular Biotechnology, Dublin City University, DublinNational Institute for Cellular Biotechnology, Dublin City University, DublinInstitute of Physiology, Medical Faculty, University of Bonn, BonnDepartment of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland; Kathleen Lonsdale Institute for Human Health Research, Maynooth University, Maynooth, Co. KildareSarcopenia of old age is characterized by the progressive loss of skeletal muscle mass and concomitant decrease in contractile strength. Age-related skeletal muscle dysfunctions play a key pathophysiological role in the frailty syndrome and can result in a drastically diminished quality of life in the elderly. Here we have used mass spectrometric analysis of the mouse hindlimb musculature to establish the muscle protein constellation at advanced age of a widely used sarcopenic animal model. Proteomic results were further analyzed by systems bioinformatics of voluntary muscles. In this report, the proteomic survey of aged muscles has focused on the expression patterns of proteins involved in the contraction-relaxation cycle, membrane cytoskeletal maintenance and the formation of the extracellular matrix. This includes proteomic markers of the fast versus slow phenotypes of myosin-containing thick filaments and actin-containing thin filaments, as well as proteins that are associated with the non-sarcomeric cytoskeleton and various matrisomal layers. The bioanalytical usefulness of the newly established reference map was demonstrated by the comparative screening of normal versus dystrophic muscles of old age, and findings were verified by immunoblot analysis. https://www.pagepressjournals.org/bam/article/view/12564Agingmass spectrometrymuscle proteomicsmuscular dystrophy |
| spellingShingle | Paul Dowling Stephen Gargan Margit Zweyer Michael Henry Paula Meleady Dieter Swandulla Kay Ohlendieck Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins located in the sarcomere, cytoskeleton and the extracellular matrix Aging mass spectrometry muscle proteomics muscular dystrophy |
| title | Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins located in the sarcomere, cytoskeleton and the extracellular matrix |
| title_full | Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins located in the sarcomere, cytoskeleton and the extracellular matrix |
| title_fullStr | Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins located in the sarcomere, cytoskeleton and the extracellular matrix |
| title_full_unstemmed | Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins located in the sarcomere, cytoskeleton and the extracellular matrix |
| title_short | Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins located in the sarcomere, cytoskeleton and the extracellular matrix |
| title_sort | proteomic reference map for sarcopenia research mass spectrometric identification of key muscle proteins located in the sarcomere cytoskeleton and the extracellular matrix |
| topic | Aging mass spectrometry muscle proteomics muscular dystrophy |
| url | https://www.pagepressjournals.org/bam/article/view/12564 |
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