Short-term arecoline exposure affected the systemic health state of mice, in which gut microbes played an important role
Arecoline is a critical bioactive component in areca nuts with toxicity and pharmacological activities. However, its effects on body health remain unclear. Here, we investigated the effects of arecoline on physiologic and biochemical parameters in mouse serum, liver, brain, and intestine. The effect...
| 發表在: | Ecotoxicology and Environmental Safety |
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| Main Authors: | , , , , , , |
| 格式: | Article |
| 語言: | 英语 |
| 出版: |
Elsevier
2023-07-01
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| 主題: | |
| 在線閱讀: | http://www.sciencedirect.com/science/article/pii/S0147651323005596 |
| _version_ | 1851906379344773120 |
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| author | Meng Xu Shunyong Su Shuaiming Jiang Wanggao Li Zeng Zhang Jiachao Zhang Xiaosong Hu |
| author_facet | Meng Xu Shunyong Su Shuaiming Jiang Wanggao Li Zeng Zhang Jiachao Zhang Xiaosong Hu |
| author_sort | Meng Xu |
| collection | DOAJ |
| container_title | Ecotoxicology and Environmental Safety |
| description | Arecoline is a critical bioactive component in areca nuts with toxicity and pharmacological activities. However, its effects on body health remain unclear. Here, we investigated the effects of arecoline on physiologic and biochemical parameters in mouse serum, liver, brain, and intestine. The effect of arecoline on gut microbiota was investigated based on shotgun metagenomic sequencing. The results showed that arecoline promoted lipid metabolism in mice, manifested as significantly reduced serum TC and TG and liver TC levels and a reduction in abdominal fat accumulation. Arecoline intake significantly modulated the neurotransmitters 5-HT and NE levels in the brain. Notably, arecoline intervention significantly increased serum IL-6 and LPS levels, leading to inflammation in the body. High-dose arecoline significantly reduced liver GSH levels and increased MDA levels, which led to oxidative stress in the liver. Arecoline intake promoted the release of intestinal IL-6 and IL-1β, causing intestinal injury. In addition, we observed a significant response of gut microbiota to arecoline intake, reflecting significant changes in diversity and function of the gut microbes. Further mechanistic exploration suggested that arecoline intake can regulate gut microbes and ultimately affect the host's health. This study provided technical help for the pharmacochemical application and toxicity control of arecoline. |
| format | Article |
| id | doaj-art-e479e8c348564e16b81ecf67ee5c6be3 |
| institution | Directory of Open Access Journals |
| issn | 0147-6513 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| spelling | doaj-art-e479e8c348564e16b81ecf67ee5c6be32025-08-19T22:03:40ZengElsevierEcotoxicology and Environmental Safety0147-65132023-07-0125911505510.1016/j.ecoenv.2023.115055Short-term arecoline exposure affected the systemic health state of mice, in which gut microbes played an important roleMeng Xu0Shunyong Su1Shuaiming Jiang2Wanggao Li3Zeng Zhang4Jiachao Zhang5Xiaosong Hu6School of Food Science and Engineering, School of public administration, Hainan University, Haikou 570228, ChinaSchool of Food Science and Engineering, School of public administration, Hainan University, Haikou 570228, ChinaSchool of Food Science and Engineering, School of public administration, Hainan University, Haikou 570228, ChinaSchool of Food Science and Engineering, School of public administration, Hainan University, Haikou 570228, ChinaSchool of Food Science and Engineering, School of public administration, Hainan University, Haikou 570228, ChinaSchool of Food Science and Engineering, School of public administration, Hainan University, Haikou 570228, China; One Health Institute, Hainan University, Haikou 570228, China; Corresponding authors at: School of Food Science and Engineering, School of public administration, Hainan University, Haikou 570228, China.School of Food Science and Engineering, School of public administration, Hainan University, Haikou 570228, China; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; Corresponding authors at: School of Food Science and Engineering, School of public administration, Hainan University, Haikou 570228, China.Arecoline is a critical bioactive component in areca nuts with toxicity and pharmacological activities. However, its effects on body health remain unclear. Here, we investigated the effects of arecoline on physiologic and biochemical parameters in mouse serum, liver, brain, and intestine. The effect of arecoline on gut microbiota was investigated based on shotgun metagenomic sequencing. The results showed that arecoline promoted lipid metabolism in mice, manifested as significantly reduced serum TC and TG and liver TC levels and a reduction in abdominal fat accumulation. Arecoline intake significantly modulated the neurotransmitters 5-HT and NE levels in the brain. Notably, arecoline intervention significantly increased serum IL-6 and LPS levels, leading to inflammation in the body. High-dose arecoline significantly reduced liver GSH levels and increased MDA levels, which led to oxidative stress in the liver. Arecoline intake promoted the release of intestinal IL-6 and IL-1β, causing intestinal injury. In addition, we observed a significant response of gut microbiota to arecoline intake, reflecting significant changes in diversity and function of the gut microbes. Further mechanistic exploration suggested that arecoline intake can regulate gut microbes and ultimately affect the host's health. This study provided technical help for the pharmacochemical application and toxicity control of arecoline.http://www.sciencedirect.com/science/article/pii/S0147651323005596ArecolineGut microbesMetagenomic analysisToxicity and pharmacological effectsSystemic health |
| spellingShingle | Meng Xu Shunyong Su Shuaiming Jiang Wanggao Li Zeng Zhang Jiachao Zhang Xiaosong Hu Short-term arecoline exposure affected the systemic health state of mice, in which gut microbes played an important role Arecoline Gut microbes Metagenomic analysis Toxicity and pharmacological effects Systemic health |
| title | Short-term arecoline exposure affected the systemic health state of mice, in which gut microbes played an important role |
| title_full | Short-term arecoline exposure affected the systemic health state of mice, in which gut microbes played an important role |
| title_fullStr | Short-term arecoline exposure affected the systemic health state of mice, in which gut microbes played an important role |
| title_full_unstemmed | Short-term arecoline exposure affected the systemic health state of mice, in which gut microbes played an important role |
| title_short | Short-term arecoline exposure affected the systemic health state of mice, in which gut microbes played an important role |
| title_sort | short term arecoline exposure affected the systemic health state of mice in which gut microbes played an important role |
| topic | Arecoline Gut microbes Metagenomic analysis Toxicity and pharmacological effects Systemic health |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323005596 |
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