Direct Testing for KPC-Mediated Carbapenem Resistance from Blood Samples Using a T2 Magnetic Resonance Based Assay

Molecular-based carbapenem resistance testing in Gram-negative bacterial bloodstream infections (BSIs) is currently limited because of the reliance on positive blood culture (BC) samples. The T2Resistance™ panel may now allow the detection of carbapenemase- and other β-lactamase encoding genes direc...

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Bibliographic Details
Published in:Antibiotics
Main Authors: Giulia De Angelis, Riccardo Paggi, Thomas J. Lowery, Jessica L. Snyder, Giulia Menchinelli, Maurizio Sanguinetti, Brunella Posteraro, Antonella Mencacci
Format: Article
Language:English
Published: MDPI AG 2021-08-01
Subjects:
antimicrobial resistance
blood sample
bloodstream infection
direct detection
KPC carbapenemase
magnetic resonance
Online Access:https://www.mdpi.com/2079-6382/10/8/950
Description
Summary:Molecular-based carbapenem resistance testing in Gram-negative bacterial bloodstream infections (BSIs) is currently limited because of the reliance on positive blood culture (BC) samples. The T2Resistance™ panel may now allow the detection of carbapenemase- and other β-lactamase encoding genes directly from blood samples. We detected carbapenem resistance genes in 11 (84.6%) of 13 samples from patients with BC-documented BSIs (10 caused by KPC-producing <i>Klebsiella</i><i>pneumoniae</i> and 1 caused by VIM/CMY-producing <i>Citrobacter freundii</i>). Two samples that tested negative for carbapenem resistance genes were from patients with BC-documented BSIs caused by KPC-producing <i>K. pneumoniae</i> who were receiving effective antibiotic therapy. In conclusion, our findings suggest that the T2Resistance™ panel can be a reliable tool for diagnosing carbapenem-resistant Gram-negative bacterial BSIs.
ISSN:2079-6382

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