Genotyping of High-risk Human Papilloma virus (HPV) among Iraqi women in Baghdad by Multiplex PCR

• 發表在: مجلة مركز بحوث التقنيات الاحيائية
• Main Authors: Ashna J. Faik Faik, Mudhafar Q. Saber Saber, Wisam J. Mohammed Mohammed, Bashar Z. Ibraheem Ibraheem, Kawther R. Lateef Lateef, Areeg S. Hassen Areeg S. Hassen
• 格式: Article
• 語言: 英语
• 出版: Al-Nahrain University/ Biotechnology Research Center 2015-01-01
• 主題: Human Papilloma Virus (HPV), RCP, cervical neoplasia Pap smear
• 在線閱讀: https://www.jobrc.org/index.php/jobrc/article/view/402
• ISSN: 1815-1140; 2708-1370

Human papilloma virus (HPV) infection is a causative factor for cervical cancer. Early detection risk HPV types might help to identify women at high risk of cervical cancer. The aim of of high HPV infection in population of rgir hgih determine the occurrence of the present study was to Iraqi women in Baghdad by using Multiplex PCR determine the percentage and genotyping of Human Papilloma Virus and to put the best prevention and control program in Iraqi women. Study started at January 2009 to March 2010, cervical samples were collected from 856 women aged 16–70. HPV DNA amplification was performed using HPV High Risk Typing PCR Kit test for qualitative detection and genotyping of HPV types 16, 18, 31, 33, 35, 39, 45, 52, 56, 58, 59, 66 in HPV was detected in 106 ( 12,38% ) of the study population, with a range of the cervical swabs. 16-70 years age groups. Results showed that the overall HPV prevalence twelve genotypes were identified, including HPV-33 (18.60%), HPV-35 (18.60%), HPV-56 (18.60%) ,HPV-39(10.85%), HPV-52 (10.08%), HPV-18 (7.75%), HPV-16 (4.65%), HPV-59 (4.65%), HPV-58(2.32%), HPV-31 (1.55%), HPV-45(1.55% ) and HPV-66( 0.77%). Of 856, 218 women was also tested by pap smear ith normal cytology was 198 ( 90.83%), 24(12.12%) of them were HPV positive, those with w, abnormal cytology was 20 (9.17 %), 5( 25%)of them was HPV positive. In this study unlike other epidemiological studies, HPV33,35,56 was the most frequent type (55.8%) in Baghdad, followed by HPV39, HPV52, HPV18, HPV16.