Time-lapse Whole-field Fluorescence Imaging of Microglia Processes Motility in Acute Mouse Hippocampal Slices and Analysis
Microglia are the resident immune cells of the central nervous system (CNS). In the last year, the improvements in the transgenic mouse technologies and imaging techniques have shed light on microglia functions under physiological conditions. Microglia continuously scan the brain parenchyma with the...
| Published in: | Bio-Protocol |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
| Published: |
Bio-protocol LLC
2019-04-01
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| Online Access: | https://bio-protocol.org/en/bpdetail?id=3220&type=0 |
| _version_ | 1850093779952861184 |
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| author | Bernadette Basilico Barbara Cortese Patrizia Ratano Silvia Di Angelantonio Davide Ragozzino |
| author_facet | Bernadette Basilico Barbara Cortese Patrizia Ratano Silvia Di Angelantonio Davide Ragozzino |
| author_sort | Bernadette Basilico |
| collection | DOAJ |
| container_title | Bio-Protocol |
| description | Microglia are the resident immune cells of the central nervous system (CNS). In the last year, the improvements in the transgenic mouse technologies and imaging techniques have shed light on microglia functions under physiological conditions. Microglia continuously scan the brain parenchyma with their highly motile processes, maintaining tissue homeostasis and participating in neuronal circuits refinement. Here, we describe a protocol that enables us to perform time-lapse imaging of microglial cells in acute hippocampal slices, making image acquisition possible on an electrophysiology rig equipped with a standard imaging system. Using this ex vivo approach, we investigated microglial processes scanning abilities under physiological condition in hippocampus. |
| format | Article |
| id | doaj-art-e4aabb7dbc7447bea8887c8a28eb0437 |
| institution | Directory of Open Access Journals |
| issn | 2331-8325 |
| language | English |
| publishDate | 2019-04-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| spelling | doaj-art-e4aabb7dbc7447bea8887c8a28eb04372025-08-20T00:07:48ZengBio-protocol LLCBio-Protocol2331-83252019-04-019810.21769/BioProtoc.3220Time-lapse Whole-field Fluorescence Imaging of Microglia Processes Motility in Acute Mouse Hippocampal Slices and AnalysisBernadette Basilico0Barbara Cortese1Patrizia Ratano2Silvia Di Angelantonio3Davide Ragozzino4Dept of Physiology and Pharmacology, Sapienza University, Rome, ItalyCNR NANOTEC-Istituto di Nanotecnologia, Dept of Physics, Sapienza University, Rome, ItalyDept of Physiology and Pharmacology, Sapienza University, Rome, ItalyCNR NANOTEC-Istituto di Nanotecnologia, Dept of Physics, Sapienza University, Rome, ItalyDept of Physiology and Pharmacology, Sapienza University, Rome, ItalyIstituto Italiano di Tecnologia, Rome, ItalyDept of Physiology and Pharmacology, Sapienza University, Rome, ItalyIRCCS Neuromed, Via Atinese, Pozzilli, ItalyMicroglia are the resident immune cells of the central nervous system (CNS). In the last year, the improvements in the transgenic mouse technologies and imaging techniques have shed light on microglia functions under physiological conditions. Microglia continuously scan the brain parenchyma with their highly motile processes, maintaining tissue homeostasis and participating in neuronal circuits refinement. Here, we describe a protocol that enables us to perform time-lapse imaging of microglial cells in acute hippocampal slices, making image acquisition possible on an electrophysiology rig equipped with a standard imaging system. Using this ex vivo approach, we investigated microglial processes scanning abilities under physiological condition in hippocampus.https://bio-protocol.org/en/bpdetail?id=3220&type=0 |
| spellingShingle | Bernadette Basilico Barbara Cortese Patrizia Ratano Silvia Di Angelantonio Davide Ragozzino Time-lapse Whole-field Fluorescence Imaging of Microglia Processes Motility in Acute Mouse Hippocampal Slices and Analysis |
| title | Time-lapse Whole-field Fluorescence Imaging of Microglia Processes Motility in Acute Mouse Hippocampal Slices and Analysis |
| title_full | Time-lapse Whole-field Fluorescence Imaging of Microglia Processes Motility in Acute Mouse Hippocampal Slices and Analysis |
| title_fullStr | Time-lapse Whole-field Fluorescence Imaging of Microglia Processes Motility in Acute Mouse Hippocampal Slices and Analysis |
| title_full_unstemmed | Time-lapse Whole-field Fluorescence Imaging of Microglia Processes Motility in Acute Mouse Hippocampal Slices and Analysis |
| title_short | Time-lapse Whole-field Fluorescence Imaging of Microglia Processes Motility in Acute Mouse Hippocampal Slices and Analysis |
| title_sort | time lapse whole field fluorescence imaging of microglia processes motility in acute mouse hippocampal slices and analysis |
| url | https://bio-protocol.org/en/bpdetail?id=3220&type=0 |
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