Development of high-resolution melting (HRM) assay to differentiate the species of Shigella isolates from stool and food samples

Abstract Shigella species, a group of intracellular foodborne pathogens, are the main causes of bacillary dysentery and shigellosis in humans worldwide. It is essential to determine the species of Shigella in outbreaks and food safety surveillance systems. The available immunological and molecular m...

Full description

Bibliographic Details
Published in:	Scientific Reports
Main Authors:	Babak Pakbin, Afshin Akhondzadeh Basti, Ali Khanjari, Wolfram Manuel Brück, Leila Azimi, Abdollah Karimi
Format:	Article
Language:	English
Published:	Nature Portfolio 2022-01-01
Online Access:	https://doi.org/10.1038/s41598-021-04484-1

Similar Items

A Multiplex High-Resolution Melting (HRM) assay to differentiate Fusarium graminearum chemotypes
by: Lovepreet Singh, et al.
Published: (2024-12-01)

A High Resolution Melting Analysis (HRM) PCR assay for the detection and identification of Old World Leishmania species.
by: Yusr Saadi-Ben Aoun, et al.
Published: (2024-12-01)

Multi-locus identification of Psilocybe cubensis by high-resolution melting (HRM)
by: Xiaochun Zhang, et al.
Published: (2021-01-01)

A Fast, Sensitive and Accurate High Resolution Melting (HRM) Technology-Based Assay to Screen for Common K-ras Mutations
by: D. Kramer, et al.
Published: (2009-01-01)

High-Resolution Melting (HRM) Curve Assay for the Identification of Eight <i>Fusarium</i> Species Causing Ear Rot in Maize
by: Simon Schiwek, et al.
Published: (2020-04-01)

Dipstick for rapid diagnosis of Shigella flexneri 2a in stool.
by: Faridabano Nato, et al.
Published: (2007-04-01)

Production of Bovine Collagen Hydrolysate with Antioxidant Activity; Optimized by Response Surface Methodology
by: Babak Pakbin, et al.
Published: (2022-10-01)

Sex determination in highly fragmented human DNA by high-resolution melting (HRM) analysis.
by: Brenda A Álvarez-Sandoval, et al.
Published: (2014-01-01)

Detection of somatic mutations by high-resolution DNA melting (HRM) analysis in multiple cancers.
by: Jesus Gonzalez-Bosquet, et al.
Published: (2011-01-01)

Distinguishing Anuran species by high‐resolution melting analysis of the COI barcode (COI‐HRM)
by: Steven Everman, et al.
Published: (2019-12-01)

APPLICATION OF HIGH-RESOLUTION MELTING (HRM) ASSAY WITH EVAGREEN DYE AS A RAPID SCREENING OF CYTOMEGALOVIRUS (CMV) DRUG RESISTANCE MUTATIONS AND ITS CHALLENGES
by: W.N.H. Wan Ghazali, et al.
Published: (2023-05-01)

Evaluation of High Resolution Melting (HRM) Analysis for Meat Species Identification of Raw and Cooked Meat
by: Peyman Gholamnezhad, et al.
Published: (2021-08-01)

High-Resolution Melting (HRM) Analysis Reveals Genotypic Differentiation of Venturia inaequalis Populations in Greece
by: Michael Chatzidimopoulos, et al.
Published: (2019-12-01)

The Effects of Nanochitosan Coating Integrated to Zataria Multiflora Boiss and Polylophium Involucratum Essential Oils on the Shelf-Life Extension of Silver Carp Fillets
by: Fatemeh Mohammadkhan, et al.
Published: (2022-08-01)

ANTIBIOTIC RESİSTANCE RATES OF Shigella spp. ISOLATED FROM STOOL SAMPLES BETWEEN 2015-2024
by: Gizem Niğdelioğlu, et al.
Published: (2024-12-01)

A Panel of High Resolution Melting (HRM) Technology-Based Assays with Direct Sequencing Possibility for Effective Mutation Screening of EGFR and K-ras Genes
by: D. A. M. Heideman, et al.
Published: (2009-01-01)

Antibiotic resistance and molecular characterization of clinical Shigella spp. isolated from hospitals located in Tehran and Qazvin cities, Iran
by: Termeh Raoufian, et al.
Published: (2025-03-01)

Detection of classic and invasive E. coli and Shigella serotypes in stools by indirect immunofluorescence
by: Waldely de Oliveira Dias, et al.
Published: (1984-12-01)

Identification and analysis of immunoreactive proteins of Shigella flexneri in human sera and stool specimens
by: Kirnpal Kaur Banga Singh, et al.
Published: (2024-05-01)

Detection and subtyping of Blastocystis sp. in human and animal stool samples using high-resolution melting analysis
by: Ghodratollah Salehi Sangani, et al.
Published: (2025-02-01)

Secretion Assay in Shigella flexneri
by: Jonathan Reinhardt, et al.
Published: (2014-11-01)

High-resolution melting (HRM) curve analysis as a potential tool for the identification of earthworm species and haplotypes
by: Anna Vaupel, et al.
Published: (2022-06-01)

Genotyping of Listeria monocytogenes isolates by high-resolution melting curve (HRM) analysis of tandem repeat locus
by: Negar Narimisa, et al.
Published: (2022-03-01)

Genotypic differentiation of Monilinia spp. populations in Serbia using a high-resolution melting (HRM) analysis
by: Antonios Zambounis, et al.
Published: (2021-03-01)

Assessment for Melting Temperature Measurement of Nucleic Acid by HRM
by: Jing Wang, et al.
Published: (2016-01-01)

Evaluation of the distribution and antibiotic resistance rates of Shigella isolates from stool samples: 5-year data
by: Betül Ceyhuni, et al.
Published: (2024-12-01)

One-Step Differential Detection of OXA-48-Like Variants Using High-Resolution Melting (HRM) Analysis
by: Min Yi Lau, et al.
Published: (2020-05-01)

PRIMARY SLX TEST USING REAL-TIME PCR BASED ON HIGH RESOLUTION MELTING (HRM) ON MICROFILARIA EXAMINATION
by: Bagus Muhammad Ihsan, et al.
Published: (2021-07-01)

An efficient method for developing SNP markers based on EST data combined with high resolution melting (HRM) analysis
by: Moriguchi Yoshinari, et al.
Published: (2010-03-01)

Prevalence, Antibiotic Resistance, Toxin-Typing and Genotyping of <i>Clostridium perfringens</i> in Raw Beef Meats Obtained from Qazvin City, Iran
by: Samaneh Hassani, et al.
Published: (2022-03-01)

Oral Abscess Caused by <i>Chryseobacterium indologenes</i> in Ball Python (<i>Python regius</i>); A Case Report
by: Iradj Ashrafi Tamai, et al.
Published: (2021-06-01)

Authentication of EU-Authorized Edible Insect Species in Food Products by DNA Barcoding and High-Resolution Melting (HRM) Analysis
by: Michaela Wildbacher, et al.
Published: (2025-02-01)

Specific Detection of Fasciola hepatica and F. gigantica in Infected Domesticated Animals Using High-Resolution Melting Analysis (HRM)
by: Zeinab MOGHADAMIZAD, et al.
Published: (2020-03-01)

Extending the Shelf Life of Fresh Camel Sausage by the Integration of Cuminum cyminum L. Essential Oil
by: Najmeh Moghimi, et al.
Published: (2021-10-01)

Molecular diagnosis of Shigella, Salmonella and Campylobacter by multiplex Real-time PCR in stool culture samples in Ouagadougou (Burkina Faso)
by: Salfo Sawadogo, et al.
Published: (2017-09-01)

Performance of liquid Amies transport medium for the recovery of Salmonella and Shigella from stool compared to molecular testing
by: Laura Collier, et al.
Published: (2025-07-01)

Barcode high-resolution melting (Bar-HRM) analysis to authenticate true cinnamon (Cinnamomum verum) from its adulterants and contaminants
by: M. A. L. M. Peiris, et al.
Published: (2025-01-01)

Barcode high-resolution melting (Bar-HRM) analysis to authenticate true cinnamon (Cinnamomum verum) from its adulterants and contaminants.
by: M A L M Peiris, et al.
Published: (2025-01-01)

Molecular profiling and sex determination of Cannabis sativa germplasm collection: Exploring microsatellite markers and high-resolution melting (HRM) analysis
by: Anastasia Boutsika, et al.
Published: (2025-08-01)

Congenital septal defects in Karachi, Pakistan: an update of mutational screening by high-resolution melting (HRM) analysis of MTHFR C677T
by: Syed Irtiza Ali, et al.
Published: (2024-01-01)