Generation of a TLR2 knockout human induced pluripotent stem cell line using CRISPR/Cas9
The TLR family plays a fundamental function in the recognition of pathogens and activation of innate immunity. TLR2 is a membrane protein that is expressed on the surface of certain cells and recognizes foreign substances; it initiates a cascade of innate immune responses. TLR2 recognizes many bacte...
| Published in: | Stem Cell Research |
|---|---|
| Main Authors: | , |
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-12-01
|
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506121004256 |
| _version_ | 1852814026508075008 |
|---|---|
| author | Hyeong-jun Han Jung-Hyun Kim |
| author_facet | Hyeong-jun Han Jung-Hyun Kim |
| author_sort | Hyeong-jun Han |
| collection | DOAJ |
| container_title | Stem Cell Research |
| description | The TLR family plays a fundamental function in the recognition of pathogens and activation of innate immunity. TLR2 is a membrane protein that is expressed on the surface of certain cells and recognizes foreign substances; it initiates a cascade of innate immune responses. TLR2 recognizes many bacterial, fungal, and viral components, as well as certain endogenous substances. We generated biallelic mutants of the TLR2 gene in human induced pluripotent stem cells (hiPSCs) using a CRISPR-Cas9 genome editing method. The TLR2 heterozygous-knockout hiPSCs retained normal morphology, gene expression, and in vivo differentiation potential. |
| format | Article |
| id | doaj-art-e4e67aa90f574340aff83fe978be59a6 |
| institution | Directory of Open Access Journals |
| issn | 1873-5061 |
| language | English |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| spelling | doaj-art-e4e67aa90f574340aff83fe978be59a62025-08-19T20:34:55ZengElsevierStem Cell Research1873-50612021-12-015710257810.1016/j.scr.2021.102578Generation of a TLR2 knockout human induced pluripotent stem cell line using CRISPR/Cas9Hyeong-jun Han0Jung-Hyun Kim1Division of Intractable Diseases, Department of Chronic Diseases Convergence Research, Korea National Institute of Health, Cheongju 28160, Republic of Korea; National Stem Cell Bank of Korea, Korea National Institute of Health, Cheongju 28160, Republic of KoreaDivision of Intractable Diseases, Department of Chronic Diseases Convergence Research, Korea National Institute of Health, Cheongju 28160, Republic of Korea; National Stem Cell Bank of Korea, Korea National Institute of Health, Cheongju 28160, Republic of Korea; Corresponding author at: Division of Intractable Diseases, Department of Chronic Diseases Convergence Research, Korea National Institute of Health, Cheongju 28160, Republic of Korea.The TLR family plays a fundamental function in the recognition of pathogens and activation of innate immunity. TLR2 is a membrane protein that is expressed on the surface of certain cells and recognizes foreign substances; it initiates a cascade of innate immune responses. TLR2 recognizes many bacterial, fungal, and viral components, as well as certain endogenous substances. We generated biallelic mutants of the TLR2 gene in human induced pluripotent stem cells (hiPSCs) using a CRISPR-Cas9 genome editing method. The TLR2 heterozygous-knockout hiPSCs retained normal morphology, gene expression, and in vivo differentiation potential.http://www.sciencedirect.com/science/article/pii/S1873506121004256 |
| spellingShingle | Hyeong-jun Han Jung-Hyun Kim Generation of a TLR2 knockout human induced pluripotent stem cell line using CRISPR/Cas9 |
| title | Generation of a TLR2 knockout human induced pluripotent stem cell line using CRISPR/Cas9 |
| title_full | Generation of a TLR2 knockout human induced pluripotent stem cell line using CRISPR/Cas9 |
| title_fullStr | Generation of a TLR2 knockout human induced pluripotent stem cell line using CRISPR/Cas9 |
| title_full_unstemmed | Generation of a TLR2 knockout human induced pluripotent stem cell line using CRISPR/Cas9 |
| title_short | Generation of a TLR2 knockout human induced pluripotent stem cell line using CRISPR/Cas9 |
| title_sort | generation of a tlr2 knockout human induced pluripotent stem cell line using crispr cas9 |
| url | http://www.sciencedirect.com/science/article/pii/S1873506121004256 |
| work_keys_str_mv | AT hyeongjunhan generationofatlr2knockouthumaninducedpluripotentstemcelllineusingcrisprcas9 AT junghyunkim generationofatlr2knockouthumaninducedpluripotentstemcelllineusingcrisprcas9 |