| الملخص: | Abstract Background Bacillin 201 has been shown to act as a biostimulant, it enhances seed germination and increases the biomass of soybean and canola plants. However, the mechanisms underlying these benefits, and their impacts on the plant-associated microbiome, remain unquantified. In this study, we investigated the causal effects of bacillin 20 on the bacterial communities associated with canola. Results We conducted a field study with three different concentrations of bacillin 20 as seed treatment and foliar spray on canola cultivar InVigor L233P followed by a randomized complete block design in clay loam and sandy loam soils and investigated how bacillin 20 influences the structure and richness of bacterial communities across biotypes, including those in root tissues and rhizosphere soil. Our findings revealed that rhizosphere soil bacterial communities clustered closely in the studied clay loam soil. Bacterial alpha diversity did not respond significantly to bacillin 20 treatments, however, Rhizobiales bacteria become most abundant in the rhizosphere soil. Also, four bacteria- Rosemicrobium sp., Sphingomonas sp. and two unclassified Planctomycetes were identified as indicator species in the microbial communities from both clay-loam and sandy-loam soils. We analysed causal relationships between the effects of bacillin 20 and experimentally controlled factors affecting bacterial communities, such as those associated with root tissue, soil and treatment (bacillin 20) types. Some bacterial taxa—ASV55 (Candidatus Xiphinematobacter) were negatively affected by bacillin 20 foliar treatment, and ASV73 (unidentified Tepidisphaerales) by seed treatment. Conclusion Overall, our study revealed key explanatory variables influencing the effect of bacillin 20 on the bacterial communities associated with canola roots and rhizosphere soil. Although soil type (clay versus sandy loam) did not directly affect the bacterial communities, we found that bacillin 20 affected the canola root system associated microbiota. This shifts in the associated bacteria could be influenced by developmental stages, not by the treatment of bacillin 20 alone.
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