A protocol for rapid and parallel isolation of myocytes and non-myocytes from multiple mouse hearts

Summary: This protocol features parallel isolation of myocytes and non-myocytes from murine hearts. It was designed with considerations for (1) time required to extract cardiac cells, (2) cell viability, and (3) protocol scalability. Here, a peristaltic pump and 3D-printed elements are combined to p...

Full description

Bibliographic Details
Published in:	STAR Protocols
Main Authors:	Gabriella E. Farrugia, Micheal A. McLellan, Kate L. Weeks, Aya Matsumoto, Charles D. Cohen, Crisdion Krstevski, Taylah L. Gaynor, Adam C. Parslow, Julie R. McMullen, Alexander R. Pinto
Format:	Article
Language:	English
Published:	Elsevier 2021-12-01
Subjects:	Cell Biology Cell isolation Cell separation/fractionation
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166721005724

Similar Items

Mapping the cellular and molecular landscape of cardiac non-myocytes in murine diabetic cardiomyopathy
by: Charles D. Cohen, et al.
Published: (2023-10-01)

Isolation, culture, and immunostaining of neonatal rat ventricular myocytes
by: Ana Helena Macedo Pereira, et al.
Published: (2021-12-01)

Frequency-dependent signaling in cardiac myocytes
by: Payam Haftbaradaran Esfahani, et al.
Published: (2022-09-01)

Protocol for simultaneous isolation of high-quality and high-quantity cardiomyocytes and non-myocyte cells from adult rat hearts
by: Alexsandra Zimmer, et al.
Published: (2024-09-01)

Direct Reprogramming of Resident Non-Myocyte Cells and Its Potential for In Vivo Cardiac Regeneration
by: Sadia Perveen, et al.
Published: (2023-04-01)

Characterization and Differentiation into Adipocytes and Myocytes of Porcine Bone Marrow Mesenchymal Stem Cells
by: Min-qing DU, et al.
Published: (2014-04-01)

Heart to heart: grafting cardiosphere‐derived cells augments cardiac self‐repair by both myocytes and stem cells
by: Jose A. Palacios, et al.
Published: (2013-02-01)

Myocyte Culture with Decellularized Skeletal Muscle Sheet with Observable Interaction with the Extracellular Matrix
by: Satoshi Nakada, et al.
Published: (2022-07-01)

Contribution of p62 to Phenotype Transition of Coronary Arterial Myocytes with Defective Autophagy
by: Junxiang Bao, et al.
Published: (2017-02-01)

In vitro transdifferentiation of umbilical cord stem cells into cardiac myocytes: Role of growth factors
by: Rasha A.M. Khattab, et al.
Published: (2013-04-01)

Generation of induced pluripotent stem cells and cardiac myocyte-like cells from a patient with noncompaction of ventricular myocardium
by: LIU Aoyi, et al.
Published: (2019-12-01)

Inhibitory effect of aloperine on transient outward potassium currents in rat cardiac myocytes
by: Xiao-Na Dong, et al.
Published: (2024-03-01)

When does the Human Heart Transition from Hyperplastic Myocyte Growth to Myocyte Hypertrophy?
by: A Martin Gerdes, et al.
Published: (2021-09-01)

SMOOTH MYOCYTES AND CARDIOVASCULAR DISEASE
by: S. S. Todorov
Published: (2009-10-01)

Tuina Promotes Repair of Chronic Cervical Muscle Injury by Regulating Satellite Cell Proliferation and Differentiation and Inhibiting Myocyte Apoptosis
by: Zhang J, et al.
Published: (2024-10-01)

Long-Term Hypoxia Negatively Influences Ca2+ Signaling in Basilar Arterial Myocytes of Fetal and Adult Sheep
by: Casey Reid, et al.
Published: (2022-01-01)

Local Control Model of a Human Ventricular Myocyte: An Exploration of Frequency-Dependent Changes and Calcium Sparks
by: Jerome Anthony E. Alvarez, et al.
Published: (2023-08-01)

Ca2+ microdomain-based excitation-transcription coupling in cardiac myocytes and vascular smooth muscle cells
by: Tsukasa Koide, et al.
Published: (2025-06-01)

Differential Effects of Beta-Hydroxybutyrate Enantiomers on Induced Pluripotent Stem Derived Cardiac Myocyte Electrophysiology
by: Matthew L. Klos, et al.
Published: (2022-10-01)

Canine Myocytes Represent a Good Model for Human Ventricular Cells Regarding Their Electrophysiological Properties
by: Péter P. Nánási, et al.
Published: (2021-07-01)

Rest potential genesis in cardiac myocytes and Purkinje fibres
by: R. I. Mkrtchyan
Published: (2008-12-01)

Mitochondria regulate proliferation in adult cardiac myocytes
by: Gregory B. Waypa, et al.
Published: (2024-07-01)

Calcium Homeostasis in Ventricular Myocytes of Diabetic Cardiomyopathy
by: Lina T. Al Kury
Published: (2020-01-01)

Studying Electric Field Effects on Embryonic Myocytes
by: Ahmed Lazrak, et al.
Published: (1997-10-01)

Jamb and jamc are essential for vertebrate myocyte fusion.
by: Gareth T Powell, et al.
Published: (2011-12-01)

Identification of the optimal reference genes for atrial fibrillation model established by iPSC-derived atrial myocytes
by: Lei Li, et al.
Published: (2024-10-01)

Single-Walled Carbon Nanotubes Inhibit TRPC4-Mediated Muscarinic Cation Current in Mouse Ileal Myocytes
by: Lina T. Al Kury, et al.
Published: (2021-12-01)

Length and PKA Dependence of Force Generation and Loaded Shortening in Porcine Cardiac Myocytes
by: Kerry S. McDonald, et al.
Published: (2012-01-01)

A multiscale computational model of spatially resolved calcium cycling in cardiac myocytes: from detailed cleft dynamics to the whole cell concentration profiles
by: Janine eVierheller, et al.
Published: (2015-09-01)

Quality Metrics for Stem Cell-Derived Cardiac Myocytes
by: Sean P. Sheehy, et al.
Published: (2014-03-01)

EFFECT OF SEVOFLURANE EXPOSURE ON ADRIAMYCIN-INDUCED INJURIES TO MYOCYTES
by: Li-hua Fan, et al.
Published: (2014-01-01)

POLB Regulates Proliferation and Apoptosis of Bovine Primary Myocytes
by: Geyang Zhang, et al.
Published: (2024-04-01)

Role of Mitochondrial ROS for Calcium Alternans in Atrial Myocytes
by: Yuriana Oropeza-Almazán, et al.
Published: (2024-01-01)

Clinical and Molecular Aspects of Iron Metabolism in Failing Myocytes
by: Bogna Kozłowska, et al.
Published: (2022-08-01)

A mathematical model of the mouse ventricular myocyte contraction.
by: Paula D Mullins, et al.
Published: (2013-01-01)

Determinants of early afterdepolarization properties in ventricular myocyte models.
by: Xiaodong Huang, et al.
Published: (2018-11-01)

EVALUATION OF THE MACROMOLECULAR STRUCTURE OF CARDIAC MYOCYTES IN PATIENTS WITH VARIOUS FORMS OF CARDIOMYOPATHY
by: A. G. Kupriyanova, et al.
Published: (2012-05-01)

Differential Regulation of MMPs, Apoptosis and Cell Proliferation by the Cannabinoid Receptors CB1 and CB2 in Vascular Smooth Muscle Cells and Cardiac Myocytes
by: Bettina Greiner, et al.
Published: (2022-12-01)

Effects of sugammadex and rocuronium on electro-mechanical activity of cardiac myocytes
by: Oguzhan Arun, et al.
Published: (2025-10-01)

Atypical myocytes: A quirky impersonator in surgical scar endometriosis!
by: Archana Shetty, et al.
Published: (2024-12-01)