Shoot Development through Modified Transverse Thin Cell Layer (tTCL) Culture of <i>Phalaenopsis</i> Hybrid Protocorms
This first-attempt study used microtome-based methods to generate a thin cell layer culture for the micropropagation of Phal. Hwafeng Redjewel × Phal. New Cinderella. Protocorms were embedded in various agarose concentrations (8–12%, <i>w</i>/<i>v</i>) and dried from 1 to 8 h...
| Published in: | Horticulturae |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
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| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/8/3/206 |
| Summary: | This first-attempt study used microtome-based methods to generate a thin cell layer culture for the micropropagation of Phal. Hwafeng Redjewel × Phal. New Cinderella. Protocorms were embedded in various agarose concentrations (8–12%, <i>w</i>/<i>v</i>) and dried from 1 to 8 h before sectioning with a microtome. Optimal conditions for slicing sections of 100 to 300 μm were achieved when the protocorms were embedded at 10% (<i>w</i>/<i>v</i>) agarose and dried for 4 h under laminar flow, and the hardness of the agarose block under these conditions reached 641.8 ± 9.5 g·cm<sup>−2</sup>. The sectioned protocorms that were cultured on an MS medium supplemented with 1.2 mg·L<sup>−1</sup> 6-benzylaminopurine and 0.1 mg·L<sup>−1</sup> α-naphthaleneacetic acid were capable of growth and differentiated through the neoformation of protocorm-like bodies (PLBs) and/or callus before subsequent regeneration into plantlets and development into healthy plants in a nursery environment. The results of this study demonstrate that microtome-based tTCL is a reliable and promising approach for mass propagation and possible virus-free propagation objectives for <i>Phalaenopsis</i>. |
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| ISSN: | 2311-7524 |