Differential expression of piRNAs in brain from patients with Alzheimer's disease

• Published in: Jichu yixue yu linchuang
• Main Author: SUN Tian-yi, LIU Fan, QIU Wen-ying, MA Chao
• Format: Article
• Language: Chinese
• Published: Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. 2021-04-01
• Subjects: alzheimer's disease|pirna|mrna
• Online Access: http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a201175.pdf

Objective To investigate the differential expression of piRNAs in brain tissues from patients with Alzheimer's disease (AD), and to study the roles of these piRNAs related to AD. Methods With age and sex matching, 6 AD (pathological AD) and 7 control subjects were selected. RNAs were converted into piRNAs after extracting from the prefrontal lobe brain tissues, then piRNAs were reversed transcription into cDNA. Based on preliminary results, 10 piRNAs expressed in brain tissues were selected: DQ571030, DQ597973, DQ576872, DQ597479, DQ586404, DQ590835, DQ600318, DQ581610, DQ586113 and DQ571669. The expression of piRNAs was detected by real-time PCR. Those mRNA related with piRNAs of significant differences were further studied. Results A trend of decrease was found in the expression of all the 10 piRNAs in the AD group. The expression of 4 piRNAs DQ597973, DQ576872, DQ597479 and DQ600318 were significantly decreased in the AD group(P＜0.05). The related mRNA LRRC37A3 of DQ600318 was further studied. DQ600318 showed the most significant difference in expression. The expression of LRRC37A3 was also significantly decreased in the AD group (P＜0.01). Conclusions The decreasing expression of DQ600318 and LRRC37A3 is potentially related to AD, and the detection of DQ600318 may function as a marker of AD pathogenesis.