Development of Dot-ELISA and Colloidal Gold Immunochromatographic Strip for Rapid and Super-Sensitive Detection of Plum Pox Virus in Apricot Trees
Plum pox virus (PPV) is a causal agent of the stone fruit tree sharka disease that often causes enormous economic losses. Due to its worldwide distribution and economic importance, rapid and reliable diagnostic technologies are becoming increasingly important for successful management of sharka dise...
| 發表在: | Viruses |
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| Main Authors: | , , , , , , , |
| 格式: | Article |
| 語言: | 英语 |
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MDPI AG
2023-01-01
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| 在線閱讀: | https://www.mdpi.com/1999-4915/15/1/169 |
| _version_ | 1851923941188173824 |
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| author | Mengmeng Guo Duo Qi Jinxi Dong Saiyu Dong Xiuling Yang Yajuan Qian Xueping Zhou Jianxiang Wu |
| author_facet | Mengmeng Guo Duo Qi Jinxi Dong Saiyu Dong Xiuling Yang Yajuan Qian Xueping Zhou Jianxiang Wu |
| author_sort | Mengmeng Guo |
| collection | DOAJ |
| container_title | Viruses |
| description | Plum pox virus (PPV) is a causal agent of the stone fruit tree sharka disease that often causes enormous economic losses. Due to its worldwide distribution and economic importance, rapid and reliable diagnostic technologies are becoming increasingly important for successful management of sharka disease. In this study, we have produced two super-sensitive and specific anti-PPV monoclonal antibodies (i.e., MAbs 13H4 and 4A11). Using these two MAbs, we have now developed a dot enzyme-linked immunosorbent assay (dot-ELISA) and a colloidal gold immunochromatographic strip (CGICS) assay. These two technologies can be used to quickly and reliably detect PPV. The results of these sensitivity assays confirmed that the dot-ELISA and CGICS assays could detect PPV infection in apricot tree leaf crude extracts diluted up to 1:5120 and 1:6400 (<i>w</i>/<i>v</i>), respectively. Further analyses using field-collected apricot tree leaf samples showed that the detection endpoint of the dot-ELISA was ~26 times above that obtained through RT-PCR, and the CGICS was as sensitive as RT-PCR. This present study is to broaden the knowledge about detection limits of dot-ELISA and CGICS for PPV monitoring. We consider that these newly developed dot-ELISA and CGICS are particularly useful for large scale PPV surveys in fields. |
| format | Article |
| id | doaj-art-e5ea846e6b904a4ca6476d8a4efe84fc |
| institution | Directory of Open Access Journals |
| issn | 1999-4915 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| spelling | doaj-art-e5ea846e6b904a4ca6476d8a4efe84fc2025-08-19T21:57:14ZengMDPI AGViruses1999-49152023-01-0115116910.3390/v15010169Development of Dot-ELISA and Colloidal Gold Immunochromatographic Strip for Rapid and Super-Sensitive Detection of Plum Pox Virus in Apricot TreesMengmeng Guo0Duo Qi1Jinxi Dong2Saiyu Dong3Xiuling Yang4Yajuan Qian5Xueping Zhou6Jianxiang Wu7State Key Laboratory of Rice Biology, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Rice Biology, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory of Rice Biology, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaPlum pox virus (PPV) is a causal agent of the stone fruit tree sharka disease that often causes enormous economic losses. Due to its worldwide distribution and economic importance, rapid and reliable diagnostic technologies are becoming increasingly important for successful management of sharka disease. In this study, we have produced two super-sensitive and specific anti-PPV monoclonal antibodies (i.e., MAbs 13H4 and 4A11). Using these two MAbs, we have now developed a dot enzyme-linked immunosorbent assay (dot-ELISA) and a colloidal gold immunochromatographic strip (CGICS) assay. These two technologies can be used to quickly and reliably detect PPV. The results of these sensitivity assays confirmed that the dot-ELISA and CGICS assays could detect PPV infection in apricot tree leaf crude extracts diluted up to 1:5120 and 1:6400 (<i>w</i>/<i>v</i>), respectively. Further analyses using field-collected apricot tree leaf samples showed that the detection endpoint of the dot-ELISA was ~26 times above that obtained through RT-PCR, and the CGICS was as sensitive as RT-PCR. This present study is to broaden the knowledge about detection limits of dot-ELISA and CGICS for PPV monitoring. We consider that these newly developed dot-ELISA and CGICS are particularly useful for large scale PPV surveys in fields.https://www.mdpi.com/1999-4915/15/1/169apricot treeplum pox virusmonoclonal antibodydot-ELISAcolloidal gold immunochromatographic stripRT-PCR |
| spellingShingle | Mengmeng Guo Duo Qi Jinxi Dong Saiyu Dong Xiuling Yang Yajuan Qian Xueping Zhou Jianxiang Wu Development of Dot-ELISA and Colloidal Gold Immunochromatographic Strip for Rapid and Super-Sensitive Detection of Plum Pox Virus in Apricot Trees apricot tree plum pox virus monoclonal antibody dot-ELISA colloidal gold immunochromatographic strip RT-PCR |
| title | Development of Dot-ELISA and Colloidal Gold Immunochromatographic Strip for Rapid and Super-Sensitive Detection of Plum Pox Virus in Apricot Trees |
| title_full | Development of Dot-ELISA and Colloidal Gold Immunochromatographic Strip for Rapid and Super-Sensitive Detection of Plum Pox Virus in Apricot Trees |
| title_fullStr | Development of Dot-ELISA and Colloidal Gold Immunochromatographic Strip for Rapid and Super-Sensitive Detection of Plum Pox Virus in Apricot Trees |
| title_full_unstemmed | Development of Dot-ELISA and Colloidal Gold Immunochromatographic Strip for Rapid and Super-Sensitive Detection of Plum Pox Virus in Apricot Trees |
| title_short | Development of Dot-ELISA and Colloidal Gold Immunochromatographic Strip for Rapid and Super-Sensitive Detection of Plum Pox Virus in Apricot Trees |
| title_sort | development of dot elisa and colloidal gold immunochromatographic strip for rapid and super sensitive detection of plum pox virus in apricot trees |
| topic | apricot tree plum pox virus monoclonal antibody dot-ELISA colloidal gold immunochromatographic strip RT-PCR |
| url | https://www.mdpi.com/1999-4915/15/1/169 |
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