PHENOTYPIC AND GENOTYPIC ANALYSIS OF A TRUEPERELLA BERNARDIAE STRAIN ISOLATED FROM A DOG

• Published in: Veterinaria
• Main Authors: Wyanda Arnafia, Siti Gusti Ningrum, Osama Sammra, Mazen Alssahen, Jörn-Peter Wickhorst, Christoph Lämmler, Ellen Prenger-Berninghoff, Markus Timke, Amir Abdulmawjood
• Format: Article
• Language: Bosnian
• Published: University of Sarajevo 2017-07-01
• Online Access: https://veterinaria-sarajevo.com/ojs/index.php/vet/article/view/269
• ISSN: 0372-6827; 2233-1360

The present study was designed to characterize phenotypically and genotypically the Trueperella bernardiae strain (T. bernardiae P5459/15) isolated from the purulent thelitis of a dog. The species identity could be confirmed by phenotypical investigations, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis, and sequencing various molecular targets. Sequencing the 16S rDNA, the β subunit of bacterial RNA polymerase encoding gene rpoBand, and the glyceraldehyde-3-phosphate dehydrogenase encoding gene gap revealed sequence similarities to the type strain T. bernardiae DSM 9152 of 99.6%, 99.3% and 99.1%, respectively. The present study is the first phenotypic and genotypic characterization of the T. bernardiae strain isolated from a dog.