Using indirect ELISA and PCR for the diagnosis of equine herpes virus-1 (EHV-1) infection in Egypt

• 出版年: Journal of Veterinary Medical Research
• 主要な著者: Fayez A. Salib, Magda A. Kalad, Hany M. Hassan, Samer F. Said
• フォーマット: 論文
• 言語: 英語
• 出版事項: Faculty of Veterinary Medicine, Beni-Suef University 2016-06-01
• 主題: ehv-1; elisa; pcr; diagnosis; egypt
• オンライン･アクセス: https://jvmr.journals.ekb.eg/article_43230_f6a625d3cbdd54280c1aafb9277a8843.pdf
• ISSN: 2357-0512; 2357-0520

The present study was carried out for studying the prevalence of equine herpesvirus-1 infection among equines in different Governorates of Egypt. One hundredeighty two equines of different ages, sexes, localities and breeds showing signs offever, respiratory manifestations, abortions, ataxia, dog sitting position, limbedema, foal depression and death were examined for the isolation of EHV-1 onboth embryonated chicken eggs and baby hamster kidney (BHK) cell culture, theaborted fetuses were histologically examined for the detection of inclusion bodies.Serum samples were collected to detect immunoglobulin-G specific to EHV-1 byusing ELISA. Nasopharyngeal swabs isolated virus and the aborted fetal tissueswere tested by PCR using specific primers to prove the infection with EHV-1. Theprevalence of EHV-1 infection in the examined animals was recorded as 4.94%. Itwas prevalent in Cairo, Giza, Kafr Elsheikh, Monofeia, El Sharkia Governorates. TheEHV-1 was isolated on the embryo chicken eggs and the pock lesion was appearedon the chorio-allantoic membranes. The cytopathic effects were also observed intissue cultures. The Liver of aborted fetuses showed necrosis of all hepatic tissueand activation of Kupffer cells with hemosiderin and the detection of eosinophilicintranuclear inclusion bodies. Indirect ELISA could detect IgG in all infected equines(n=9) from which EHV-1 isolated. PCR proved the infection with EHV-1 in theaborted fetal (n=3) tissues and gave similar results by using the horsesnasopharyngeal swabs isolated virus where 489 bp PCR products were detected inboth. In conclusion, EHV-1 is prevalent in different Governorates in Egypt. EHV-1infection could be diagnosed by intranuclear eosinophilic inclusion bodies in theaborted fetal tissues. The indirect ELISA could diagnose EHV-1 infection in all agesand sexes groups. PCR applied on aborted fetal tissues is better for the diagnosis ofEHV-1 infection than that based on the horse nasopharyngeal swabs isolated virusdue to saving time and money.