Chromosomal location of 45S rDNA and dfr gene in Citrus sinensis
Dihydroflavonol 4-reductase (DFR) is a key enzyme in the anthocyanin biosynthesis. In this study, the localization of 45S rDNA and dfr gene (named as CsDFR-bo) of the blood orange (Citrus sinensis Osbeck cv. Ruby) on chromosomes was investigated by fluorescence in situ hybridization (FISH). A karyot...
| 出版年: | Biologia Plantarum |
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| 主要な著者: | , , |
| フォーマット: | 論文 |
| 言語: | 英語 |
| 出版事項: |
Institute of Experimental Botany of the Czech Academy of Sciences
2010-12-01
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| 主題: | |
| オンライン･アクセス: | https://bp.ueb.cas.cz/artkey/bpl-201004-0038_Chromosomal-location-of-45S-rDNA-and-dfr-gene-in-Citrus-sinensis.php |
| _version_ | 1849896720784162816 |
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| author | X. Lu W. Zhou F. Gao |
| author_facet | X. Lu W. Zhou F. Gao |
| author_sort | X. Lu |
| collection | DOAJ |
| container_title | Biologia Plantarum |
| description | Dihydroflavonol 4-reductase (DFR) is a key enzyme in the anthocyanin biosynthesis. In this study, the localization of 45S rDNA and dfr gene (named as CsDFR-bo) of the blood orange (Citrus sinensis Osbeck cv. Ruby) on chromosomes was investigated by fluorescence in situ hybridization (FISH). A karyotype of C. sinensis was reconstructed based on the length of mitotic metaphase chromosomes. The 45S rDNAs were localized on chromosomes 2p and 7q. The detection ratios of 45S rDNA in the two chromosomes were 69.5 and 77.3 %, respectively. The CsDFR-bo was proved to be a single-copy gene and localized on chromosome 3p. The detection ratio of CsDFR-bo was 8.2 %. |
| format | Article |
| id | doaj-art-e6490a82d90f44828c720da5aec3cdfa |
| institution | Directory of Open Access Journals |
| issn | 1573-8264 |
| language | English |
| publishDate | 2010-12-01 |
| publisher | Institute of Experimental Botany of the Czech Academy of Sciences |
| record_format | Article |
| spelling | doaj-art-e6490a82d90f44828c720da5aec3cdfa2025-08-20T01:01:18ZengInstitute of Experimental Botany of the Czech Academy of SciencesBiologia Plantarum1573-82642010-12-0154479880010.1007/s10535-010-0146-4bpl-201004-0038Chromosomal location of 45S rDNA and dfr gene in Citrus sinensisX. Lu0W. Zhou1F. Gao2School of Life Sciences, South China Normal University, Guangzhou, P.R. ChinaSchool of Life Sciences, South China Normal University, Guangzhou, P.R. ChinaSchool of Life Sciences, South China Normal University, Guangzhou, P.R. ChinaDihydroflavonol 4-reductase (DFR) is a key enzyme in the anthocyanin biosynthesis. In this study, the localization of 45S rDNA and dfr gene (named as CsDFR-bo) of the blood orange (Citrus sinensis Osbeck cv. Ruby) on chromosomes was investigated by fluorescence in situ hybridization (FISH). A karyotype of C. sinensis was reconstructed based on the length of mitotic metaphase chromosomes. The 45S rDNAs were localized on chromosomes 2p and 7q. The detection ratios of 45S rDNA in the two chromosomes were 69.5 and 77.3 %, respectively. The CsDFR-bo was proved to be a single-copy gene and localized on chromosome 3p. The detection ratio of CsDFR-bo was 8.2 %.https://bp.ueb.cas.cz/artkey/bpl-201004-0038_Chromosomal-location-of-45S-rDNA-and-dfr-gene-in-Citrus-sinensis.phpanthocyanin biosynthesisdihydroflavonol 4-reductase genefluorescence in situ hybridization |
| spellingShingle | X. Lu W. Zhou F. Gao Chromosomal location of 45S rDNA and dfr gene in Citrus sinensis anthocyanin biosynthesis dihydroflavonol 4-reductase gene fluorescence in situ hybridization |
| title | Chromosomal location of 45S rDNA and dfr gene in Citrus sinensis |
| title_full | Chromosomal location of 45S rDNA and dfr gene in Citrus sinensis |
| title_fullStr | Chromosomal location of 45S rDNA and dfr gene in Citrus sinensis |
| title_full_unstemmed | Chromosomal location of 45S rDNA and dfr gene in Citrus sinensis |
| title_short | Chromosomal location of 45S rDNA and dfr gene in Citrus sinensis |
| title_sort | chromosomal location of 45s rdna and dfr gene in citrus sinensis |
| topic | anthocyanin biosynthesis dihydroflavonol 4-reductase gene fluorescence in situ hybridization |
| url | https://bp.ueb.cas.cz/artkey/bpl-201004-0038_Chromosomal-location-of-45S-rDNA-and-dfr-gene-in-Citrus-sinensis.php |
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