Sets of covariant residues modulate the activity and thermal stability of GH1 β-glucosidases.

The statistical coupling analysis of 768 β-glucosidases from the GH1 family revealed 23 positions in which the amino acid frequencies are coupled. The roles of these covariant positions in terms of the properties of β-glucosidases were investigated by alanine-screening mutagenesis using the fall arm...

Full description

Bibliographic Details
Published in:	PLoS ONE
Main Authors:	Fábio K Tamaki, Larissa C Textor, Igor Polikarpov, Sandro R Marana
Format:	Article
Language:	English
Published:	Public Library of Science (PLoS) 2014-01-01
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24804841/?tool=EBI

Similar Items

Mutations close to a hub residue affect the distant active site of a GH1 β-glucosidase.
by: Valquiria P Souza, et al.
Published: (2018-01-01)

Impact of Changes in Buffer Ionic Concentration and Mutations on a GH1 β‑Glucosidase Homodimer
by: Rafael S. Chagas, et al.
Published: (2025-08-01)

Tris inhibits a GH1 β-glucosidase by a linear mixed inhibition mechanism.
by: Rafael S Chagas, et al.
Published: (2025-01-01)

Mechanism of imidazole inhibition of a GH1 β‐glucosidase
by: Rafael S. Chagas, et al.
Published: (2023-05-01)

Search for independent (β/α)4 subdomains in a (β/α)8 barrel β-glucosidase.
by: Vitor M Almeida, et al.
Published: (2018-01-01)

In Silico Approach to Elucidate Factors Associated with GH1 β-Glucosidase Thermostability
by: Amer Ahmed, et al.
Published: (2019-12-01)

Structural and Functional Insights Into CmGH1, a Novel GH39 Family β-Glucosidase From Deep-Sea Bacterium
by: Yanfang Shen, et al.
Published: (2019-12-01)

In Silico Analysis of a GH3 β-Glucosidase from <i>Microcystis aeruginosa</i> CACIAM 03
by: Gustavo Marques Serra, et al.
Published: (2023-04-01)

Efficient Biotransformation of Icariin to Baohuoside I Using Two Novel GH1 β-Glucosidases
by: Xiaoling Zhang, et al.
Published: (2024-11-01)

Unveiling a classical mutant in the context of the GH3 β-glucosidase family in Neurospora crassa
by: Yuxin Zhang, et al.
Published: (2024-01-01)

Cloning, expression and characterization of an ethanol tolerant GH3 β-glucosidase from Myceliophthora thermophila
by: Anthi Karnaouri, et al.
Published: (2013-02-01)

A thermostable and highly active fungal GH3 β-glucosidase generated by random and saturation mutagenesis
by: Chiaki MATSUZAKI, et al.
Published: (2025-02-01)

A Recombinant Thermophilic and Glucose-Tolerant GH1 β-Glucosidase Derived from Hehua Hot Spring
by: Qian Zhu, et al.
Published: (2024-02-01)

A novel GH3-β-glucosidase from soda lake metagenomic libraries with desirable properties for biomass degradation
by: Oliyad Jeilu, et al.
Published: (2024-05-01)

Recombinant GH3 β-glucosidase stimulated by xylose and tolerant to furfural and 5-hydroxymethylfurfural obtained from Aspergillus nidulans
by: Diandra de Andrades, et al.
Published: (2024-07-01)

Cloning, purification, and characterization of GH3 β-glucosidase, MtBgl85, from Microbulbifer thermotolerans DAU221
by: Hyo-Min Pyeon, et al.
Published: (2019-07-01)

Deciphering domain structures of Aspergillus and Streptomyces GH3-β-Glucosidases: a screening system for enzyme engineering and biotechnological applications
by: Andika Sidar, et al.
Published: (2024-09-01)

Identification and molecular characterization of a psychrophilic GH1 β-glucosidase from the subtropical soil microorganism Exiguobacterium sp. GXG2
by: Bangqiao Yin, et al.
Published: (2019-10-01)

Stability of commercial glucanase and β-glucosidase preparations under hydrolysis conditions
by: Oscar Rosales-Calderon, et al.
Published: (2014-06-01)

Characterization of a novel cold-adapted GH1 β-glucosidase from Psychrobacillus glaciei and its application in the hydrolysis of soybean isoflavone glycosides
by: Jinjian He, et al.
Published: (2024-01-01)

Characterization of a thermophilic and glucose-tolerant GH1 β-glucosidase from hot springs and its prospective application in corn stover degradation
by: Yu-Ying Huang, et al.
Published: (2023-12-01)

Chitosan‐Based metal-organic framework for Stabilization of β-glucosidase: Reusability and storage stability
by: Mustafa Zeyadi, et al.
Published: (2023-11-01)

Characterization, thermostable mechanism, and molecular docking of a novel glucose-tolerant β-glucosidase/β-galactosidase from the GH1 family isolated from Rehai hot spring
by: Yu-Ying Huang, et al.
Published: (2025-04-01)

Characterization of BrGH3A, a bovine rumen-derived glycoside hydrolase family 3 β-glucosidase with a permuted domain arrangement.
by: Phiraya Pitchayatanakorn, et al.
Published: (2024-01-01)

Characterization of BrGH3A, a bovine rumen-derived glycoside hydrolase family 3 β-glucosidase with a permuted domain arrangement
by: Phiraya Pitchayatanakorn, et al.
Published: (2024-01-01)

Characterization of a Novel Hyperthermophilic GH1 β-Glucosidase from <i>Acidilobus</i> sp. and Its Application in the Hydrolysis of Soybean Isoflavone Glycosides
by: Jinjian He, et al.
Published: (2024-03-01)

Structure and Function of BcpE2, the Most Promiscuous GH3-Family Glucose Scavenging Beta-Glucosidase
by: Benoit Deflandre, et al.
Published: (2022-08-01)

Multifunctional elastin-like polypeptide renders β-glucosidase enzyme phase transition and high stability
by: Yang Zhou, et al.
Published: (2019-06-01)

α-Glucosidase and β-glucosidase from psychrotrophic strain arthrobacter sp. C2-2
by: Eva Benešová, et al.
Published: (2005-06-01)

Computational Studies on the Potency and Selectivity of PUGNAc Derivatives Against GH3, GH20, and GH84 β-N-acetyl-D-hexosaminidases
by: Lili Dong, et al.
Published: (2019-04-01)

Microstructure stability of GH4169 and GH4169C alloys with high content of phosphorus and niobium
by: NI Tong-wei, et al.
Published: (2016-09-01)

Natural variation in the GhTTL promoter modulates cotton fiber elongation through the GhTALE–GhTTL–GhBIN2 module
by: Le Liu, et al.
Published: (2025-10-01)

Comparative Investigations on Different β-Glucosidase Surrogate Substrates
by: Felix Marcel Rainer Graf, et al.
Published: (2022-02-01)

In vivo assay to identify bacteria with β-glucosidase activity
by: Erwin Strahsburger, et al.
Published: (2017-11-01)

β-Glucosidase genes differentially expressed during composting
by: Xinyue Zhang, et al.
Published: (2020-10-01)

On the Decomposition of Covariate Shift Assumption for the Set-to-Set Matching
by: Masanari Kimura
Published: (2023-01-01)

Characterization of the interdependency between residues that bind the substrate in a β-glycosidase
by: M.H. Tomassi, et al.
Published: (2010-01-01)

Correction to “Detection of Active Mammalian GH31 α‑Glucosidases in Health and Disease Using In-Class, Broad-Spectrum Activity-Based Probes”
by: Jianbing Jiang, et al.
Published: (2017-05-01)

Targeted metatranscriptomics of compost-derived consortia reveals a GH11 exerting an unusual exo-1,4-β-xylanase activity
by: Bruno L. Mello, et al.
Published: (2017-11-01)

Effect of Residual Nb on Mechanical Properties of GH4080A Alloy
by: Zhang Hong, et al.
Published: (2025-01-01)