Coordinated alternation of DNA methylation and alternative splicing of PBRM1 affect bovine sperm structure and motility
DNA methylation and gene alternative splicing drive spermatogenesis. In screening DNA methylation markers and transcripts related to sperm motility, semen from three pairs of full-sibling Holstein bulls with high and low motility was subjected to reduced representation bisulphite sequencing. A total...
| 出版年: | Epigenetics |
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| 主要な著者: | , , , , , , , , , , , , , , |
| フォーマット: | 論文 |
| 言語: | 英語 |
| 出版事項: |
Taylor & Francis Group
2023-12-01
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| 主題: | |
| オンライン･アクセス: | http://dx.doi.org/10.1080/15592294.2023.2183339 |
| _version_ | 1850301730896478208 |
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| author | Chunhong Yang Yao Xiao Xiuge Wang Xiaochao Wei Jinpeng Wang Yaping Gao Qiang Jiang Zhihua Ju Yaran Zhang Wenhao Liu Ning Huang Yanqin Li Yundong Gao Lingling Wang Jinming Huang |
| author_facet | Chunhong Yang Yao Xiao Xiuge Wang Xiaochao Wei Jinpeng Wang Yaping Gao Qiang Jiang Zhihua Ju Yaran Zhang Wenhao Liu Ning Huang Yanqin Li Yundong Gao Lingling Wang Jinming Huang |
| author_sort | Chunhong Yang |
| collection | DOAJ |
| container_title | Epigenetics |
| description | DNA methylation and gene alternative splicing drive spermatogenesis. In screening DNA methylation markers and transcripts related to sperm motility, semen from three pairs of full-sibling Holstein bulls with high and low motility was subjected to reduced representation bisulphite sequencing. A total of 948 DMRs were found in 874 genes (gDMRs). Approximately 89% of gDMR-related genes harboured alternative splicing events, including SMAD2, KIF17, and PBRM1. One DMR in exon 29 of PBRM1 with the highest 5mC ratio was found, and hypermethylation in this region was related to bull sperm motility. Furthermore, alternative splicing events at exon 29 of PBRM1 were found in bull testis, including PBRM1-complete, PBRM1-SV1 (exon 28 deletion), and PBRM1-SV2 (exons 28–29 deletion). PBRM1-SV2 exhibited significantly higher expression in adult bull testes than in newborn bull testes. In addition, PBRM1 was localized to the redundant nuclear membrane of bull sperm, which might be related to sperm motility caused by sperm tail breakage. Therefore, the hypermethylation of exon 29 may be associated with the production of PBRM1-SV2 in spermatogenesis. These findings indicated that DNA methylation alteration at specific loci could regulate gene splicing and expression and synergistically alter sperm structure and motility. |
| format | Article |
| id | doaj-art-e693d1b5000c4b249c10c7260cefa468 |
| institution | Directory of Open Access Journals |
| issn | 1559-2294 1559-2308 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| spelling | doaj-art-e693d1b5000c4b249c10c7260cefa4682025-08-19T23:30:53ZengTaylor & Francis GroupEpigenetics1559-22941559-23082023-12-0118110.1080/15592294.2023.21833392183339Coordinated alternation of DNA methylation and alternative splicing of PBRM1 affect bovine sperm structure and motilityChunhong Yang0Yao Xiao1Xiuge Wang2Xiaochao Wei3Jinpeng Wang4Yaping Gao5Qiang Jiang6Zhihua Ju7Yaran Zhang8Wenhao Liu9Ning Huang10Yanqin Li11Yundong Gao12Lingling Wang13Jinming Huang14Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural SciencesInstitute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural SciencesInstitute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural SciencesInstitute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural SciencesInstitute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural SciencesInstitute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural SciencesInstitute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural SciencesInstitute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural SciencesInstitute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural SciencesInstitute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural SciencesInstitute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural SciencesInstitute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural SciencesInstitute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural SciencesInstitute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural SciencesInstitute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural SciencesDNA methylation and gene alternative splicing drive spermatogenesis. In screening DNA methylation markers and transcripts related to sperm motility, semen from three pairs of full-sibling Holstein bulls with high and low motility was subjected to reduced representation bisulphite sequencing. A total of 948 DMRs were found in 874 genes (gDMRs). Approximately 89% of gDMR-related genes harboured alternative splicing events, including SMAD2, KIF17, and PBRM1. One DMR in exon 29 of PBRM1 with the highest 5mC ratio was found, and hypermethylation in this region was related to bull sperm motility. Furthermore, alternative splicing events at exon 29 of PBRM1 were found in bull testis, including PBRM1-complete, PBRM1-SV1 (exon 28 deletion), and PBRM1-SV2 (exons 28–29 deletion). PBRM1-SV2 exhibited significantly higher expression in adult bull testes than in newborn bull testes. In addition, PBRM1 was localized to the redundant nuclear membrane of bull sperm, which might be related to sperm motility caused by sperm tail breakage. Therefore, the hypermethylation of exon 29 may be associated with the production of PBRM1-SV2 in spermatogenesis. These findings indicated that DNA methylation alteration at specific loci could regulate gene splicing and expression and synergistically alter sperm structure and motility.http://dx.doi.org/10.1080/15592294.2023.2183339alternative splicingdairy cattleepigenetic regulationpbrm1spermatogenesis |
| spellingShingle | Chunhong Yang Yao Xiao Xiuge Wang Xiaochao Wei Jinpeng Wang Yaping Gao Qiang Jiang Zhihua Ju Yaran Zhang Wenhao Liu Ning Huang Yanqin Li Yundong Gao Lingling Wang Jinming Huang Coordinated alternation of DNA methylation and alternative splicing of PBRM1 affect bovine sperm structure and motility alternative splicing dairy cattle epigenetic regulation pbrm1 spermatogenesis |
| title | Coordinated alternation of DNA methylation and alternative splicing of PBRM1 affect bovine sperm structure and motility |
| title_full | Coordinated alternation of DNA methylation and alternative splicing of PBRM1 affect bovine sperm structure and motility |
| title_fullStr | Coordinated alternation of DNA methylation and alternative splicing of PBRM1 affect bovine sperm structure and motility |
| title_full_unstemmed | Coordinated alternation of DNA methylation and alternative splicing of PBRM1 affect bovine sperm structure and motility |
| title_short | Coordinated alternation of DNA methylation and alternative splicing of PBRM1 affect bovine sperm structure and motility |
| title_sort | coordinated alternation of dna methylation and alternative splicing of pbrm1 affect bovine sperm structure and motility |
| topic | alternative splicing dairy cattle epigenetic regulation pbrm1 spermatogenesis |
| url | http://dx.doi.org/10.1080/15592294.2023.2183339 |
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