Serendipitous Discovery of Desert Hairy Scorpion Mitogenomes as Bycatch in Venom Data via Nanopore Sequencing
While originally intending to explore the venom gland microbiome of the desert hairy scorpion <i>Hadrurus arizonensis</i> Ewing, 1928, nanopore sequencing serendipitously recovered complete mitochondrial genomes for this iconic arachnid. Phylogenetic analysis of these high-quality genome...
| Published in: | Arthropoda |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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MDPI AG
2024-04-01
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| Subjects: | |
| Online Access: | https://www.mdpi.com/2813-3323/2/2/9 |
| _version_ | 1850371365441372160 |
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| author | Matthew R. Graham Carlos E. Santibáñez-López Jessica R. Zehnpfennig Dylan S. Tillman Barbara Murdoch |
| author_facet | Matthew R. Graham Carlos E. Santibáñez-López Jessica R. Zehnpfennig Dylan S. Tillman Barbara Murdoch |
| author_sort | Matthew R. Graham |
| collection | DOAJ |
| container_title | Arthropoda |
| description | While originally intending to explore the venom gland microbiome of the desert hairy scorpion <i>Hadrurus arizonensis</i> Ewing, 1928, nanopore sequencing serendipitously recovered complete mitochondrial genomes for this iconic arachnid. Phylogenetic analysis of these high-quality genomes places <i>Hadrurus</i> as sister to <i>Uroctonus</i>, in agreement with some phylogenomic hypotheses. Additionally, we reveal significant genetic variation among individuals from the same population, highlighting the potential of mitogenomics for population genetics and phylogeography. This study showcases the effectiveness and affordability of nanopore sequencing for research with non-model organisms, opening new avenues for investigating arachnid biodiversity, evolution, and biogeography. |
| format | Article |
| id | doaj-art-e731eb63c8b940e99020ca93a12e81dd |
| institution | Directory of Open Access Journals |
| issn | 2813-3323 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| spelling | doaj-art-e731eb63c8b940e99020ca93a12e81dd2025-08-19T23:01:14ZengMDPI AGArthropoda2813-33232024-04-012211912910.3390/arthropoda2020009Serendipitous Discovery of Desert Hairy Scorpion Mitogenomes as Bycatch in Venom Data via Nanopore SequencingMatthew R. Graham0Carlos E. Santibáñez-López1Jessica R. Zehnpfennig2Dylan S. Tillman3Barbara Murdoch4Department of Biology, Eastern Connecticut State University, Willimantic, CT 06226, USADepartment of Biology, Western Connecticut State University, Danbury, CT 06810, USADepartment of Biology, Central Michigan University, Mount Pleasant, MI 48859, USADepartment of Biology, Eastern Connecticut State University, Willimantic, CT 06226, USADepartment of Biology, Eastern Connecticut State University, Willimantic, CT 06226, USAWhile originally intending to explore the venom gland microbiome of the desert hairy scorpion <i>Hadrurus arizonensis</i> Ewing, 1928, nanopore sequencing serendipitously recovered complete mitochondrial genomes for this iconic arachnid. Phylogenetic analysis of these high-quality genomes places <i>Hadrurus</i> as sister to <i>Uroctonus</i>, in agreement with some phylogenomic hypotheses. Additionally, we reveal significant genetic variation among individuals from the same population, highlighting the potential of mitogenomics for population genetics and phylogeography. This study showcases the effectiveness and affordability of nanopore sequencing for research with non-model organisms, opening new avenues for investigating arachnid biodiversity, evolution, and biogeography.https://www.mdpi.com/2813-3323/2/2/9bycatchgene orderHadruridaeHadrurinaeMinIONONT |
| spellingShingle | Matthew R. Graham Carlos E. Santibáñez-López Jessica R. Zehnpfennig Dylan S. Tillman Barbara Murdoch Serendipitous Discovery of Desert Hairy Scorpion Mitogenomes as Bycatch in Venom Data via Nanopore Sequencing bycatch gene order Hadruridae Hadrurinae MinION ONT |
| title | Serendipitous Discovery of Desert Hairy Scorpion Mitogenomes as Bycatch in Venom Data via Nanopore Sequencing |
| title_full | Serendipitous Discovery of Desert Hairy Scorpion Mitogenomes as Bycatch in Venom Data via Nanopore Sequencing |
| title_fullStr | Serendipitous Discovery of Desert Hairy Scorpion Mitogenomes as Bycatch in Venom Data via Nanopore Sequencing |
| title_full_unstemmed | Serendipitous Discovery of Desert Hairy Scorpion Mitogenomes as Bycatch in Venom Data via Nanopore Sequencing |
| title_short | Serendipitous Discovery of Desert Hairy Scorpion Mitogenomes as Bycatch in Venom Data via Nanopore Sequencing |
| title_sort | serendipitous discovery of desert hairy scorpion mitogenomes as bycatch in venom data via nanopore sequencing |
| topic | bycatch gene order Hadruridae Hadrurinae MinION ONT |
| url | https://www.mdpi.com/2813-3323/2/2/9 |
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