Characterization of <i>Prunus Necrotic Ringspot Virus</i> and <i>Cherry Virus</i> A Infecting Myrobalan Rootstock

• Published in: Viruses
• Main Authors: Karima Ben Mansour, Petr Komínek, Marcela Komínková, Jana Brožová
• Format: Article
• Language: English
• Published: MDPI AG 2023-08-01
• Subjects: PNRSV; CVA; phylogenetic analysis; biological assay; symptoms
• Online Access: https://www.mdpi.com/1999-4915/15/8/1723
• ISSN: 1999-4915

<i>Prunus necrotic ringspot virus</i> (PNRSV) and <i>cherry virus</i> A (CVA) are two viruses that mainly infect plants of the genus <i>Prunus</i>. Full-length sequences of these two viruses, collected in the Czech Republic from <i>Prunus cerasifera</i> plants, were obtained via HTS sequencing. Phylogenetic analyses based on the NJ method and Splitstree tools showed that the Czech PNRSV isolate (ON088600-ON088602) is a divergent isolate from other molecular groups, sharing less than 97% pairwise nucleotide identity with members of other groups. The Czech CVA isolate (ON088603) belonged to molecular subgroup III-2, clustered with isolates from non-cherry hosts, and shared the highest pairwise nucleotide identity (99.7%) with an isolate of Australian origin.