| Summary: | Guided implant placement has been shown to be more accurate than free-handed insertion. Still, implant position deviations occur and could possibly pose risks. Thus, there is a quest to identify factors that might impair the accuracy of implantation protocols using templates. This study aimed to investigate the influence of autoclaving cycles (cycle 1: 121 °C, 1 bar, 20.5 min; cycle 2: 134 °C, 2 bar, 5.5 min) on the Vickers hardness and flexural modulus of five different materials used for 3D-printed insertion guides. The specimens were subjected to Vickers hardness tests, showing significant changes in the Vickers hardness for two and three materials out of five for cycle 1 and 2, respectively. The results of the three-point bending tests (<i>n =</i> 15 specimens per material) showed decreasing flexural moduli after autoclaving. However, changes were significant only for one material, which presented a significant decrease in the flexural modulus after cycle 2. No significant changes were detected after cycle 1. In conclusion, our findings show that autoclaving can alter the mechanical properties of the templates to some extent, especially with cycle 2. Whether these modifications are associated with dimensional changes of the templates and reduced accuracy of the implantation protocols remains to be investigated.
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