| Summary: | Transitory starch plays a central role in the life cycle of plants. Many aspects of this important metabolism remain unknown; however, starch granules provide insight into this persistent metabolic process. Therefore, monitoring alterations in starch granules with high temporal resolution provides one significant avenue to improve understanding. Here, a previously established method that combines LCSM and safranin-O staining for in vivo imaging of transitory starch granules in leaves of <i>Arabidopsis thaliana</i> was employed to demonstrate, for the first time, the alterations in starch granule size and morphology that occur both throughout the day and during leaf aging. Several starch-related mutants were included, which revealed differences among the generated granules. In <i>ptst2</i> and <i>sex1-8</i>, the starch granules in old leaves were much larger than those in young leaves; however, the typical flattened discoid morphology was maintained. In <i>ss4</i> and <i>dpe2/phs1/ss4</i>, the morphology of starch granules in young leaves was altered, with a more rounded shape observed. With leaf development, the starch granules became spherical exclusively in <i>dpe2/phs1/ss4</i>. Thus, the presented data provide new insights to contribute to the understanding of starch granule morphogenesis.
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