The influence of sulfur limitation on expression levels of an o-acetylserine (thiol) lyase gene cloned from Vicia sativa
The o-acetylserine (thiol) lyase (OAS-TL) gene is part of the plant sulfate assimilation pathway. The synthesis of cysteine, the first product of the pathway containing organic sulfur, has been previously observed to be dependent on the availability of sulfur and activity of OAS-TL. In this study, t...
| Published in: | Biologia Plantarum |
|---|---|
| Main Authors: | , |
| Format: | Article |
| Language: | English |
| Published: |
Institute of Experimental Botany of the Czech Academy of Sciences
2010-09-01
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| Subjects: | |
| Online Access: | https://bp.ueb.cas.cz/artkey/bpl-201003-0031_The-influence-of-sulfur-limitation-on-expression-levels-of-an-o-acetylserine-thiol-lyase-gene-clo.php |
| _version_ | 1850337889414545408 |
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| author | A. U. Novero R. Ford |
| author_facet | A. U. Novero R. Ford |
| author_sort | A. U. Novero |
| collection | DOAJ |
| container_title | Biologia Plantarum |
| description | The o-acetylserine (thiol) lyase (OAS-TL) gene is part of the plant sulfate assimilation pathway. The synthesis of cysteine, the first product of the pathway containing organic sulfur, has been previously observed to be dependent on the availability of sulfur and activity of OAS-TL. In this study, the ability of Vicia sativa L. to alter its metabolism to compensate for deficiencies brought about by sulfur stress was utilized to elucidate the functionality of Voas-tl5 (GenBank Accession No. DQ456491), a gene cloned from Vicia sativa. The transcription levels of Voas-tl5 increased in response to sulfur deficiency, indicating that the gene was active in the cysteine biosynthetic pathway. |
| format | Article |
| id | doaj-art-e7e2be9544514d88995d3b5f6f91a86d |
| institution | Directory of Open Access Journals |
| issn | 1573-8264 |
| language | English |
| publishDate | 2010-09-01 |
| publisher | Institute of Experimental Botany of the Czech Academy of Sciences |
| record_format | Article |
| spelling | doaj-art-e7e2be9544514d88995d3b5f6f91a86d2025-08-19T23:15:26ZengInstitute of Experimental Botany of the Czech Academy of SciencesBiologia Plantarum1573-82642010-09-0154357958210.1007/s10535-010-0104-1bpl-201003-0031The influence of sulfur limitation on expression levels of an o-acetylserine (thiol) lyase gene cloned from Vicia sativaA. U. Novero0R. Ford1Melbourne School of Land and Environment, University of Melbourne, Parkville, AustraliaMelbourne School of Land and Environment, University of Melbourne, Parkville, AustraliaThe o-acetylserine (thiol) lyase (OAS-TL) gene is part of the plant sulfate assimilation pathway. The synthesis of cysteine, the first product of the pathway containing organic sulfur, has been previously observed to be dependent on the availability of sulfur and activity of OAS-TL. In this study, the ability of Vicia sativa L. to alter its metabolism to compensate for deficiencies brought about by sulfur stress was utilized to elucidate the functionality of Voas-tl5 (GenBank Accession No. DQ456491), a gene cloned from Vicia sativa. The transcription levels of Voas-tl5 increased in response to sulfur deficiency, indicating that the gene was active in the cysteine biosynthetic pathway.https://bp.ueb.cas.cz/artkey/bpl-201003-0031_The-influence-of-sulfur-limitation-on-expression-levels-of-an-o-acetylserine-thiol-lyase-gene-clo.phpcysteine biosynthesissulfate assimilationvetch |
| spellingShingle | A. U. Novero R. Ford The influence of sulfur limitation on expression levels of an o-acetylserine (thiol) lyase gene cloned from Vicia sativa cysteine biosynthesis sulfate assimilation vetch |
| title | The influence of sulfur limitation on expression levels of an o-acetylserine (thiol) lyase gene cloned from Vicia sativa |
| title_full | The influence of sulfur limitation on expression levels of an o-acetylserine (thiol) lyase gene cloned from Vicia sativa |
| title_fullStr | The influence of sulfur limitation on expression levels of an o-acetylserine (thiol) lyase gene cloned from Vicia sativa |
| title_full_unstemmed | The influence of sulfur limitation on expression levels of an o-acetylserine (thiol) lyase gene cloned from Vicia sativa |
| title_short | The influence of sulfur limitation on expression levels of an o-acetylserine (thiol) lyase gene cloned from Vicia sativa |
| title_sort | influence of sulfur limitation on expression levels of an o acetylserine thiol lyase gene cloned from vicia sativa |
| topic | cysteine biosynthesis sulfate assimilation vetch |
| url | https://bp.ueb.cas.cz/artkey/bpl-201003-0031_The-influence-of-sulfur-limitation-on-expression-levels-of-an-o-acetylserine-thiol-lyase-gene-clo.php |
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