Effects of Alprostadil on experimental peritoneal fibrosis in rats

• Published in: Linchuang shenzangbing zazhi
• Main Authors: ZHANG Yan-min, CHEN Wei-dong, WAN Sheng, CHEN Dan, CHEN Fang, DING Yan-qiong, WANG Zeng-si, TIAN Hong-dan, XIONG Fei
• Format: Article
• Language: Chinese
• Published: Editorial Department of Journal of Clinical Nephrology 2015-01-01
• Subjects: Peritoneal fibrosis; Alprostadil; Transforming growth factor-β; Toll like receptor 4
• Online Access: http://www.lcszb.com/thesisDetails?columnId=57920730&Fpath=home&index=0
• ISSN: 1671-2390

Objective To investigate the effects and possible action mechanism of Alprostadil in the treatment of experimental peritoneal fibrosis in rats.Methods The rat peritoneal fibrosis model was induced by high glucose dialysate and lipopolysaccharide(LPS).The rats were randomly divided into five groups:control group,model group,low-dose Alprostadil-treated group,middle-dose Alprostadil-treated group and high-dose Alprostadil-treated group.The Alprostadil-treated groups received daily intraperitoneal injection of different doses of Alprostadil.Six weeks later all rats were anaesthetized.The peritoneal membrane function was investigated by peritoneal equilibration test(PET).The abdominal membrane was stained with HE and Masson method.The levels of TGF-β and TLR4 in peritoneum were detected by immunohistochemical method.Results The number of vascularity in model group was greater than that in Alprostadil-treated groups.The concentrations of TGFβand TLR4 in blood and dialysate were also decreased obviously in Alprostadil-treated groups.The levels of TGFβ and TLR4 in peritoneumin in Alprostadil-treated groups were significantly lower than those in model group.Conclusions Alprostadil are beneficial to the prevention and treatment of peritoneal fibrosis in experimental model rats.