Optimization of a thermochemical antibody stripping method for enhanced tyramide signal amplification-based opal multiplex immunohistochemistry in fragile tissues

Abstract Objective Tyramide signal amplification (TSA)-induced Opal multiplex immunohistochemistry (mIHC) represents an advanced methodology for the in-situ detection of multiple target proteins. A critical aspect of this technique is the complete removal of primary and secondary antibodies to preve...

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Bibliographic Details
Published in:	BMC Research Notes
Main Authors:	Xiaotong Yu, Yan Song, Li Chen, Lixiang Xue, Jianling Yang, Chen Huang
Format:	Article
Language:	English
Published:	BMC 2025-05-01
Subjects:	Multiplexed immunohistochemistry Antibody stripping Tyramide signal amplification Hybridization oven-based antibody removing (HO-AR)
Online Access:	https://doi.org/10.1186/s13104-025-07301-4

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