3′-End cDNA Pool Suitable for Differential Display from a Small Number of Cells

We have generated a 3′ cDNA pool from the RNA of only 1000 or fewer cells by reverse transcription (RT) from an extended oligo(dT) primer with a 3′ degenerate base and a second strand primer with four degenerate 3′bases, followed by PCR. Reproducible differential displays (DD) can be made from this...

书目详细资料
发表在:	BioTechniques
Main Authors:	S. Zhao, G. Molnar, J. Zhang, L. Zheng, L. Averboukh, A.B. Pardee
格式:	文件
语言:	英语
出版:	Taylor & Francis Group 1998-05-01
在线阅读:	https://www.future-science.com/doi/10.2144/98245rr01

相似书籍

Detection of Eukaryotic cDNA in Differential Display Is Enhanced by the Addition of E. coli RNA
由: H. Melichar, et al.
出版: (2000-01-01)

Three Methods for Identification of True Positive Cloned cDNA Fragments in Differential Display
由: Shanchuan Zhao, et al.
出版: (1996-03-01)

Better Gel Resolution and Longer cDNAs Increase the Precision of Differential Display
由: L. Averboukh, et al.
出版: (1996-05-01)

Differential Display Probes for cDNA Arrays
由: Thomas Trenkle, et al.
出版: (1999-09-01)

Increased Efficiency in Screening Large Numbers of cDNA Fragments Generated by Differential Display
由: J. Christopher Corton, et al.
出版: (1997-05-01)

Elimination of False Positives Generated Through PCR Re-amplification of Differential Display cDNA
由: G. Miele, et al.
出版: (1998-07-01)

Phage display of cDNA libraries: enrichment of cDNA expression using open reading frame selection
由: Peggy Ho Faix, et al.
出版: (2004-06-01)

Conversion of cDNA differential display results (DDRT-PCR) into quantitative transcription profiles
由: Koopmann Birger, et al.
出版: (2005-04-01)

Full-Length cDNA Cloning and Determination of mRNA 5′and 3′Ends by Amplification of Adaptor-Ligated cDNA
由: A. Chenchik, et al.
出版: (1996-09-01)

System for PCR Identification of cDNA Ends (SPICE)
由: David R. Koehler, et al.
出版: (1999-07-01)

Preparation of Phage Display cDNA Libraries for Identifying Immunogenic Tumor Antigens: Challenges in Functional cDNA Presentation and Approaches to Overcoming Them
由: Nuša Brišar, et al.
出版: (2024-11-01)

Rolling circle amplification-RACE: a method for simultaneous isolation of 5′ and 3′ cDNA ends from amplified cDNA templates
由: Alexios N. Polidoros, et al.
出版: (2006-07-01)

Construction of cDNA Libraries for Highly Efficient DNA Sequencing from the 3′ End of Expressed Genes
由: Glenn K. Fu, et al.
出版: (2003-04-01)

De-novo transcriptome sequencing of a normalized cDNA pool from influenza infected ferrets.
由: Jeremy V Camp, et al.
出版: (2012-01-01)

Indirect measurements of differential gene expression with cDNA microarrays
由: Thomas J. Belbin, et al.
出版: (2004-02-01)

Technical analysis of cDNA microarrays.
由: Cinda P Scott, et al.
出版: (2009-01-01)

3’ Rapid Amplification of cDNA Ends (3’ RACE) Using Arabidopsis Samples
由: Encarnación Rodríguez-Cazorla, et al.
出版: (2015-10-01)

LEDGF/p75 Deficiency Increases Deletions at the HIV-1 cDNA Ends
由: Murilo T. D. Bueno, et al.
出版: (2017-09-01)

High-Stringency Subtraction for the Identification of Differentially Regulated cDNA Clones
由: Charles P. Scutt, et al.
出版: (1997-09-01)

PCR-Amplified cDNA Probes for Verification of Differentially Expressed Genes
由: Ralf Ross, et al.
出版: (1997-05-01)

cDNA Microarray Analysis in Hepatocyte Differentiation in Huh 7 Cells
由: Yo-Ichi Yamashita M.D. Ph.D., et al.
出版: (2004-10-01)

An RNA Binding Peptide Consisting of Four Types of Amino Acid by in Vitro Selection Using cDNA Display
由: Shigefumi Kumachi, et al.
出版: (2016-07-01)

Differential display of flower bud mRNA of genic male sterility (GMS) AB line in Chinese cabbage-pak-choi and analysis of differential cDNA fragments
由: CAO Jia-shu, et al.
出版: (2001-11-01)

PCR Amplification of cDNA Libraries for Cloning and Screening
由: Taek Hyon You, et al.
出版: (1998-04-01)

Optimized Rapid Amplification of cDNA Ends (RACE) for Mapping Bacterial mRNA Transcripts
由: Daniel Tillett, et al.
出版: (2000-03-01)

Improved Method for Detecting Differentially Expressed Genes Using cDNA Indexing
由: C.J. Shaw-Smith, et al.
出版: (2000-05-01)

Combination of PCR Subtraction and cDNA Microarray for Differential Gene Expression Profiling
由: Michael T. Beck, et al.
出版: (2001-10-01)

Localizing triplet periodicity in DNA and cDNA sequences
由: Stein Lincoln D, et al.
出版: (2010-11-01)

Identification and Verification of Differential Display cDNAs Using Gene-Specific Primers and Hybridization Arrays
由: K.J. Martin, et al.
出版: (1998-06-01)

Differential representation of sunflower ESTs in enriched organ-specific cDNA libraries in a small scale sequencing project
由: Heinz Ruth A, et al.
出版: (2003-09-01)

Generation of Full-Length cDNA Libraries Enriched for Differentially Expressed Genes for Functional Genomics
由: B. Zhumabayeva, et al.
出版: (2001-03-01)

RNA Amplification Strategies for cDNA Microarray Experiments
由: J. Wang, et al.
出版: (2003-02-01)

Isolation of Full-Length cDNA Clones Using SMARTTM cDNA and a Biotin-Streptavidin Bead System
由: Vincent Ciavatta, et al.
出版: (2000-09-01)

Normalization of a cDNA Library Cloned in λZAP by a Long PCR and cDNA Reassociation Procedure
由: K. Fischer, et al.
出版: (2003-02-01)

cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method
由: Chathuni Jayathilake, et al.
出版: (2019-12-01)

5’ Rapid Amplification of cDNA Ends (5’ RACE) of Agrobacterial T-DNA Genes within Transformed Plant Sample
由: Yi Zhang
出版: (2015-09-01)

Simultaneous amplification of 5′ and 3′ cDNA ends based on template-switching effect and inverse PCR
由: Jun-Chao Huang, et al.
出版: (2006-02-01)

Simple Strategy for Sequencing cDNA Clones
由: Ling-Wen Zeng, et al.
出版: (1996-09-01)

Strategy for the Design of Custom cDNA Microarrays
由: Matthias G.O. Lorenz, et al.
出版: (2003-06-01)

Comparison of standard exponential and linear techniques to amplify small cDNA samples for microarrays
由: von Arnold Sara, et al.
出版: (2005-05-01)