| Summary: | (1) Background: Marker-based optical motion tracking is the gold standard in gait analysis; however, markerless solutions are rapidly emerging today. Algorithms like Openpose can track human movement from a video. Few studies have assessed the validity of this method. This study aimed to assess the reliability of Openpose in measuring the kinematics and spatiotemporal gait parameters. (2) Methods: This analysis used simultaneously recorded video and optoelectronic motion capture data. We assessed 20 subjects with different gait impairments (healthy, right hemiplegia, left hemiplegia, paraparesis). The two methods were compared using computing absolute errors (AEs), intraclass correlation coefficients (ICCs), and cross-correlation coefficients (CCs) for normalized gait cycle joint angles. (3) Results: The spatiotemporal parameters showed an ICC between good to excellent, and the absolute error was very small: cadence AE = 1.63 step/min, Mean Velocity AE = 0.16 m/s. The Range of Motion (ROM) showed a good to excellent agreement in the sagittal plane. Furthermore, the normalized gait cycle CCC values indicated moderate to strong coupling in the sagittal plane. (4) Conclusions: We found Openpose to be accurate for sagittal plane gait kinematics and for spatiotemporal gait parameters in the healthy and pathological subjects assessed.
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