Diisoprenyl Cyclohexene-Type Meroterpenoids with Cytotoxic Activity from a Mangrove Endophytic Fungus <i>Aspergillus</i> sp. GXNU-Y85

• Published in: Marine Drugs
• Main Authors: Feng Qin, Zi-Shuo Song, Li Luo, Xiang-Long Bo, Fu-Rong Wu, Mei-Jing Tan, Fan-Fan Wang, Xi-Shan Huang, Heng-Shan Wang
• Format: Article
• Language: English
• Published: MDPI AG 2024-01-01
• Subjects: mangrove endophytic fungus; <i>Aspergillus</i> sp.; meroterpenoids; anti-cancer activity
• Online Access: https://www.mdpi.com/1660-3397/22/2/58
• ISSN: 1660-3397

Five new diisoprenyl cyclohexene-type meroterpenoids, aspergienynes J–N (<b>1</b>–<b>5</b>), along with three known analogues (<b>6</b>–<b>8</b>), were obtained from the mangrove endophytic fungal strain <i>Aspergillus</i> sp. GXNU-Y85. The chemical structures, including their absolute configurations, were established via spectroscopic data and comparison of experimental and calculated ECD spectra. Cytotoxicity assay results indicated that compound <b>8</b> had strong cytotoxicity against HeLa cancer cells, and its IC₅₀ value was 11.8 μM. In addition, flow cytometry analysis revealed that the cytotoxicity of <b>8</b> was due to the induction of G1 cell cycle arrest and apoptosis in HeLa cells.