| Summary: | The <i>Hosta</i> hybrid cultivar ‘So Sweet’, an important ornamental and widely used horticultural plant, is noted for its rich, fragrant white flowers. The main aroma components of <i>Hosta</i> flowers are terpenoids, mainly monoterpenes. Until now, the terpene synthases responsible for terpene production in <i>Hosta</i> were not described. In this study, two terpene synthase (TPS) genes (Hs<i>TPS1</i> and Hs<i>TPS2</i>) were cloned and characterized to further study their function. Furthermore, the volatile terpenes of <i>Hosta</i> ’So Sweet’ in two flower development stages from two in vitro enzyme tests were analyzed by gas chromatography–mass spectrometry (GC–MS). We analyzed the expression levels of two genes at four different developmental stages using quantitative real-time PCR, while localization was analyzed using <i>Nicotina benthamiana</i> leaves. In vitro, the two proteins were identified to mainly produce linalool and nerol. In addition, the active products of the two recombinant proteins were (<i>E,E</i>)-farnesol and <i>(E,E</i>)-farnesal, respectively, using farnesyl pyrophosphate as a substrate. The high expression of Hs<i>TPS1</i> and Hs<i>TPS2</i> was correlated with the release of components of <i>Hosta</i> flowers. To our knowledge, this is the first time that the terpene synthase genes of <i>Hosta</i> species have been isolated and identified, providing an opportunity to study the terpene metabolic pathways in <i>Hosta</i> species.
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