Evaluation of Methods to Assess in vivo Activity of Engineered Genome-Editing Nucleases in Protoplasts

Genome-editing is being implemented in increasing number of plant species using engineered sequence specific nucleases (SSNs) such as Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated systems (CRISPR/Cas9), Transcription activator like effector nucleases (TALENs), and more...

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Bibliographic Details
Published in:	Frontiers in Plant Science
Main Authors:	Satya Swathi Nadakuduti, Colby G. Starker, Dae Kwan Ko, Thilani B. Jayakody, C. Robin Buell, Daniel F. Voytas, David S. Douches
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2019-02-01
Subjects:	genome-editing CRISPR/Cas9 TALENs protoplasts double-stranded oligodeoxynucleotides NHEJ
Online Access:	https://www.frontiersin.org/article/10.3389/fpls.2019.00110/full

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